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  1. #1
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    Kudzu Root Extract Research- Anti-Estrogen

    Was doing some research on the ingredient Daidzin in Legal Gears anti-e , and came across this, sounds interesting, would like to hear some of the experts opinions on this:

    ==============================================
    Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.

    Boue SM, Wiese TE, Nehls S, Burow ME, Elliott S, Carter-Wientjes CH, Shih BY, McLachlan JA, Cleveland TE.

    Agricultural Research Service, Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@nola.srrc.usda.gov

    Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.

    PMID: 12670155 [PubMed - indexed for MEDLINE]
    ==============================================

    -Ski
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    Originally Posted by Skigazzi View Post
    Was doing some research on the ingredient Daidzin in Legal Gears anti-e , and came across this, sounds interesting, would like to hear some of the experts opinions on this:

    ==============================================
    Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.

    Boue SM, Wiese TE, Nehls S, Burow ME, Elliott S, Carter-Wientjes CH, Shih BY, McLachlan JA, Cleveland TE.

    Agricultural Research Service, Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@nola.srrc.usda.gov

    Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.

    PMID: 12670155 [PubMed - indexed for MEDLINE]
    ==============================================

    -Ski
    This is interesting...I was researching Kudzu for its historical use as a deterrent for alcohol use. This could also be an effect worth looking into.
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  3. #3
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    Originally Posted by VegetaGD View Post
    This is interesting...I was researching Kudzu for its historical use as a deterrent for alcohol use. This could also be an effect worth looking into.
    Strong bump!

    Might be worth reposting this study in the Supplement Science section for a better response?
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    Bent NOT Broken pushinweightwi's Avatar
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    we sell solgars thai kudzu for menopausal symptoms and it work better than any other phyto estrogen out there.
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    Bump again. Reps to Skigazzi for posting this.
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    Originally Posted by Skigazzi View Post
    Was doing some research on the ingredient Daidzin in Legal Gears anti-e , and came across this, sounds interesting, would like to hear some of the experts opinions on this:

    ==============================================
    Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.

    Boue SM, Wiese TE, Nehls S, Burow ME, Elliott S, Carter-Wientjes CH, Shih BY, McLachlan JA, Cleveland TE.

    Agricultural Research Service, Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@nola.srrc.usda.gov

    Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.

    PMID: 12670155 [PubMed - indexed for MEDLINE]
    ==============================================

    -Ski
    Doesn't , Kudzu extract - according to that study - display pro-estrogenic properties and not anti-estrogenic?
    ThermoLife scientific consultant
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  7. #7
    Geordie Boot Boy Robboe's Avatar
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    Originally Posted by VegetaGD View Post
    This is interesting...I was researching Kudzu for its historical use as a deterrent for alcohol use. This could also be an effect worth looking into.
    Wow, nice novel concept. Any idea how that works?
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    Originally Posted by DR_P View Post
    Doesn't , Kudzu extract - according to that study - display pro-estrogenic properties and not anti-estrogenic?
    Not sure whether it is pro-estrogen, but it certainly appears to have estrogen-like effect. Based on this abstract alone i don't know what benefit it would have in an anti-estrogen product.
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  9. #9
    Registered User VegetaGD's Avatar
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    Originally Posted by Robboe View Post
    Wow, nice novel concept. Any idea how that works?
    http://harvardscience.harvard.edu/me...ol-consumption

    Here's the rub...I'm not sure I have enough research yet to determine if it acts in an estrogenic or anti-estrogenic manner.

    That said, it's proven that abusing alcohol can wreak havoc on your T to E ratio so perhaps it's the lesser of two evils.

    I'm pretty sure that a healthy portion of people posting on these boards are social drinkers, and if using this before going out can turn a 12 drink night into a 5 drink night, it's been a huge win in my opinion.
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    Registered User VegetaGD's Avatar
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    Originally Posted by pushinweightwi View Post
    we sell solgars thai kudzu for menopausal symptoms and it work better than any other phyto estrogen out there.
    Have you any experience with men using it?
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    Registered User VegetaGD's Avatar
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    Originally Posted by Skigazzi View Post
    Was doing some research on the ingredient Daidzin in Legal Gears anti-e , and came across this, sounds interesting, would like to hear some of the experts opinions on this:

    ==============================================
    Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.

    Boue SM, Wiese TE, Nehls S, Burow ME, Elliott S, Carter-Wientjes CH, Shih BY, McLachlan JA, Cleveland TE.

    Agricultural Research Service, Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@nola.srrc.usda.gov

    Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.

    PMID: 12670155 [PubMed - indexed for MEDLINE]
    ==============================================

    -Ski
    Here is a study suggesting Kudzu acts as an AI (Puerarin is an isoflavone in Kudzu).

    http://www.ncbi.nlm.nih.gov/pubmed/1...ubmed_RVDocSum
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    Can Anything Be Done About Estrogen Dominance?

    The incidence of breast cancer in the United States is 141.1 and the incidence of breast cancer in Northern Thailand(Chiang Mai Province) is 13.7. Most of the males and females in this province consume a phytoestrogen herbal substance called Pueraria mirifica PM. This plant has a tuberous root which can be eaten. The plant appears identical to other local plants and can only be distinguished from other non-PM containing plants during the 2 weeks annually when it is flowering. Most of the PM being sold is misidentified and has none of the benefits of PM.

    PM contains a valuable phytoestrogen named miroestrol. Miroestrol is similar to the safest human estrogen estriol. Miroestrol is 3000 times stronger than the estrogenic activity exhibited by soy isoflavones(genistein). PM has more than 1000 times the estrogenic activity of red clover. In addition to miroestrol PM contains substantial amounts of other phytoestrogens including daidzein, genistein, puerarin, and mirificin.

    When miroestrol enters an estrogen receptor it modulates the effect on the receptor. PM supplements when the estrogenic activity is low and dilutes if the receptor estrogenic activity is high. Miroestrol has no effect on estrogen levels in the body as it only influences the estrogen receptor.

    In a clinical study on menopausal women in Thailand miroestrol lowered hot flashes 11 fold and night sweats 4 fold within 30 days. Most women start to notice relief of symptoms within one week.

    Approximately 70% of Japanese women taking PM noted an increase in breast size. This was more apparent in younger women than older women.

    The dosage of PM in females was 2 mg. per kilo of body weight. In the first month of therapy for menopausal symptoms 3 mg can be taken. Each pill of Beyond Hormone Replacement Therapy HRT contains about 80 mg. so taking 3 pills would supply this. A U.S. woman weighing 70 kilos should take one pill 80 mg. twice daily. In the clinical studies in Thailand doses no toxicity was noted even in doses 2000 times the usual dosage. Traditional Thai usage has menstruating women skip the therapy during menses.

    Therapy With PM In Males

    The dosage in males is one half that of women (1mg. per kilo of body weight). Males have not noticed any increase in breast size. Both males and females in this region of Northern Thailand continue to be physically active and retain their black hair into their 80s. Elderly women have firm breasts and youthful appearing skin. Elderly men continue to be sexually active. Their memories are good and they have not been ill. One man in his 80s was surprised when asked if he got up to urinate during the night. He replied ďNo one here wakes up to do that.Ē

    H.R.T. Plus

    Dr. Gary Gordonís company Longevity Plus has formulated a new sublingual product that contains Pauraria Mirifica phytoestrogen complex along with Methylcobalamin and Nutri-Folate which has all forms of folic acid.

    Methylcobalamin became available in 1998 and quickly gained recognition for itís ability to regenerate nerves. Research has shown that sublingual Vitamin B12 in this product Methylcobalamin MC was nearly as effective as injections of B12. Additionally protection can be obtained against the development of diabetic polyneuropathy, reversal of Bellís palsy, improvement in chronic depression, reduction in the frequency of disabling headaches and improvement in neurologic conditions. This form of B12 has improved testicular function allowing some patients to again become sexually potent.

    Some patients in psychiatric institutions have been restored to normal life when their B12 deficiency is corrected. B12 therapy can also stimulate return of normal growth patterns in children, Patients who are pregnant, using birth control pills and all vegetarians are at risk for B12 deficiency.

    Deleterious high homocysteine blood levels are quickly reversed by MC therapy which may also help prevent Alzheimerís Disease, aging, cancer and arteriosclerosis. Folic Acid deficiency is very common currently. Alcohol destroys stores of folic acid. As many as 50% of pregnant women are at risk of folic acid depletion which can cause spina bifida, cleft palate, brain damage and impaired learning in the newborn child.



    Folic acid depleted individuals are vulnerable to viral, fungal and bacterial infections. Nutri-Folate contains all the forms of folic acid including natural 5íMTHF. Vitamin B6 can reverse carpal tunnel syndrome, helps prevent problems caused by excessive sugar intake and improves utilization of protein.

    The dose of H.R.T. Plus is one or two tablets taken sublingually daily. Swallow after the tablet has dissolved. This therapy provides the valuable estrogenic substance PM along with Methylcobalamin and all the valuable forms of folic acid.
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    Why is this thread implying anti-e effects when it would seem like it has pro-e effects? Does not make sense to me.
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    Originally Posted by kissdadookie View Post
    Why is this thread implying anti-e effects when it would seem like it has pro-e effects? Does not make sense to me.
    It turns off the receptors
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    Originally Posted by Italymodel22 View Post
    It turns off the receptors
    But it would appear that one would end up with an overall increase in e by using it or does the phytoestrogens not matter?
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