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  1. #1
    Registered User marek's Avatar
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    l-arginine found in NO2

    from www.supplementwatch.com
    Arginine

    Description Arginine is an amino acid and is also referred to as "L-Arginine" (the L stands for "levo" and designates the amino acid as naturally occurring and distinguishes from the D or " " synthetic amino acids.).

    Claims Protection from heart disease
    Reduces cholesterol
    Lowers blood pressure
    Improves poor circulation


    Theory Arginine is a key component of the nitric oxide pathway ? and important cascade of reactions involved in vasodilation and related to cardiovascular function. Arginine supplements have been associated with reductions in symptoms associated with coronary artery disease and may be capable of slowing the progression of atherosclerosis
    In the body, arginine serves as the substrates for the nitric oxide synthase enzyme, which catalyzes the oxidation of arginine to produce citrulline and nitric oxide (NO). In the cells that line the blood vessels (endothelium cells), nitric oxide production causes vasodilation (opening of the vessels). NO is involved in the overall regulation of systemic vascular resistance, where it inhibits the adherence of cells and foreign substances to the blood vessel walls and helps suppress the overgrowth of smooth muscle cells in the lining of the vessels.

    Because humans can synthesize arginine, it has been classified as a non-essential amino acid. Recent evidence suggests that the rate of synthesis of arginine in the body is insufficient for optimal health ? a situation which would re-classify arginine as a semi-essential or conditionally essential amino acid.


    Scientific Support In people with elevated cholesterol levels, it is common to see a reduced ability of the endothelium to produce NO and, therefore, to dilate effectively. In addition, because NO production may be limited, blood cells such as monocytes and platelets are more likely to attach themselves to the inner vessel wall and lead to blockages. Arginine supplements (8-21 grams per day) have been shown to restore endothelial vasodilation in the coronary arteries of people with high cholesterol and reduce the ability of blood cells to adhere to the vessel walls. Improvements in coronary artery blood flow and reductions in myocardial ischemia and walking pain due to claudication have been noted with arginine supplements (9-14 g/day).

    Safety Arginine supplements have been used safely in patients with heart disease in doses up to more than 20 grams per day.

    Value For those individuals at risk for coronary artery disease, including those who experience ischemia due to reduced blood flow and oxygen delivery, arginine supplements may be an effective strategy for improving circulation to the heart and other affected areas (such as vessels in the calves).

    Dosage A daily arginine requirement has been calculated to be approximately 8 grams per day (based on calculations for a 70-kg person). Since the average American diet contains only about 5 grams of arginine per day, there would appear to be a deficit in intake versus requirements. Importantly, the primary dietary sources of arginine, like all amino acids, are meats and other high protein foods (nuts, eggs).
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  2. #2
    Registered User phaledax's Avatar
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    (book mark post)
    good post
    Last edited by phaledax; 05-28-2004 at 11:23 AM.
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    Registered User LJ57's Avatar
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    Is the arginine AKG in products like NO2 any better than regular L-arginine?
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  4. #4
    soon to be huge TylerT's Avatar
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    NO2 or Nitrous

    Does anyone know if nitrous is better than NO2 or NOX3 , ive heard it was but i wanted to make sure
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    Re: NO2 or Nitrous

    Originally posted by TylerT
    Does anyone know if nitrous is better than NO2 or NOX3 , ive heard it was but i wanted to make sure
    well look for some studies showing Di-L-Arginine malate is superior to AAKG, which will be very very hard to find, so why not just taker user feedback and try out Nitrous. I had at least 10x better results with it than NO2
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  6. #6
    soon to be huge TylerT's Avatar
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    Nitrous

    All of these posts about Nitrous just seem to be saying that it gives you better pumps and more indurance for sets. Does it build muscle also, I havent heard anyone say anything about getting bigger with it...
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  7. #7
    Supplement Connosueir kendog's Avatar
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    Well if it give you more endurance for sets than it would make you bigger.
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    Getswole Guru DAMBIGlsu's Avatar
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    Ornithine helps prompt the release of growth hormone, which promotes the metabolism of excess body fat. This effect is enhanced if ornithine is combined with arginine.
    Arginine as great as it is for numerous reason should not be taken in the high doses for long periods of time that companys recommend. A recent study found that several weeks of large doses may result in thickening and coarsening of the skin. This is understandable because arginine is found in high concentrations in the skin and connective tissues. Also studies show that high doses of Arginine-AKG can be damaging to the brain if taken for long periods of time. My recommendation along with several doctors specializing in sports nutrition is taking does of 3-4grams 30-45 minutes prior to training. This raise your nitric-oxide levels for about 3-4 hours which is ampel time. Taking it throughout the day does keep you in a constant "pumped-up" state but what is the need for that when you only benefit from it while you are in the gym and immediately afterwards. Companies merely tell you to take so much so they can rake in profits. Save yourself some money and try this on for size.
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    Originally posted by DAMBIGlsu
    Ornithine helps prompt the release of growth hormone, which promotes the metabolism of excess body fat.
    yea this was a HOT old school supp, slowly making a slow slow comeback, but what dose is it effective/noticable.
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  10. #10
    Registered User 97S281_27's Avatar
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    Originally posted by zachattack43
    yea this was a HOT old school supp, slowly making a slow slow comeback, but what dose is it effective/noticable.
    I've noitced a difference when i supplement 500mg of ornithine for every 1000mg arginine. Some other health based products made by now have a ratio simliar to this just smaller amounts. It also depends upon your diet, if you can eat foods higher in these particular amino's you don't have to supplement as much, or the more you supplement even better result you'll get.
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  11. #11
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    Originally posted by 97S281_27
    I've noitced a difference when i supplement 500mg of ornithine for every 1000mg arginine. Some other health based products made by now have a ratio simliar to this just smaller amounts. It also depends upon your diet, if you can eat foods higher in these particular amino's you don't have to supplement as much, or the more you supplement even better result you'll get.
    but what foods are high in ornithine???
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  12. #12
    C6H13NO2 pu12en12g's Avatar
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    Whatever you think of Nitric Oxide products, this is worth the read:

    http://www.blackwell-synergy.com/lin....11605.x/full/

    "Arginine is a basic amino acid that plays several pivotal roles in cellular physiology. Like any amino acid, it is involved with protein synthesis, but it is also intimately involved with cell signaling through the production of nitric oxide and cell proliferation through its metabolism to ornithine and the other polyamines. Because of these multiple functions, arginine is an essential substrate for wound healing processes. Numerous studies have shown that arginine supplementation can lead to normalization or improvement of healing. This article reviews the basic biochemistry and cell signaling within which arginine performs its functions. In particular, the requirement for this amino acid in tissue repair is highlighted."

    "Arginine is a dibasic amino acid. It is a constituent of several proteins in the body, and its metabolism is intimately tied to several metabolic pathways involved in the synthesis of urea, NO, polyamines, agmatine, and creatine phosphate. Arginine can be provided via nutritional intake or via new synthesis. Citrulline, generated from glutamine in the small intestine, is the major precursor for arginine. 4 About 50% of the ingested arginine is released into the portal circulation. The other part is directly utilized in the small bowel. The physiological uptake of arginine and citrulline by the liver is low because the liver does not express large amounts of the cationic transporter for the basic amino acid arginine (system y+). Therefore, most of the portal venous arginine and citrulline enters the systemic circulation and serves as substrate for extrahepatic tissues."

    "...Although iNOS gene deletion delays, and arginine and NO administration improve healing, the exact mechanisms of action of NO on wound healing parameters are still unknown."

    "One of the well-known biological effects of arginine is its very potent secretagogue activity on the pituitary 4547 and pancreatic glands. 48,49 The beneficial effects of supplemental arginine on wound healing are similar to the effects noted when growth hormone is administered to wounded animals or burned children."

    "The response of healthy elderly human volunteers given 2 weeks of dietary supplementation with 30 g of arginine aspartate is enhanced wound collagen accumulation together with a significant elevation in circulating IGF-1. 39 This suggests that arginine does stimulate pituitary activity in the doses used and that this may be one of the mechanisms of action underlying its beneficial effect on wound healing."
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  13. #13
    Registered User Ceaze's Avatar
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    Originally posted by DAMBIGlsu
    Ornithine helps prompt the release of growth hormone, which promotes the metabolism of excess body fat. This effect is enhanced if ornithine is combined with arginine.
    the increase in GH is of little significance
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  14. #14
    C6H13NO2 pu12en12g's Avatar
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    "Nitric oxide generation from L-arginine is required for optimal human peripheral blood lymphocyte DNA synthesis."- Efron DT, Kirk SJ, Regan MC, Wasserkrug HL, Barbul A. Department of Surgery, Sinai Hospital of Baltimore, MD 21215.

    "These data suggest that nitric oxide synthesis is critical to wound collagen accumulation and acquisition of mechanical strength".

    Nitric oxide regulates wound healing - J Surg Res. 1996 Jun;63(1):237-40. Schaffer MR, Tantry U, Gross SS, Wasserburg HL, Barbul A.

    "NO has been shown to be cytostatic to multiple cell types including endothelial cells, smooth muscle cells, hepatocytes, and fibroblasts [37, 38 and 39]. Depending on the cell type this effect can be cGMP dependent [40] or independent [41]. Target enzymes include complexes I and II of the respiratory chain [42] and ribonucleotide reductase [43], a rate-limiting enzyme in the DNA synthetic pathway. NO is cytostatic in large doses. However, several studies have shown that NO can stimulate cell proliferation when added in low concentrations [44 and 45]. Recent evidence suggests that NO can also reduce cell proliferation by inhibiting ornithine-decarboxylase activity, the rate limiting enzyme for polyamine formation [46].

    NO also regulates gene expression [47 and 48] and cellular differentiation [49 and 50]. Regulation of gene expression by NO probably occurs indirectly, through amplification of other regulatory mechanisms [51]. For example, although NO is critical for wound collagen deposition, clear-cut enhancement of collagen synthesis or gene expression has not been found (see the following). Collagen metabolism and accumulation are tightly regulated through the activity of collagenases and their inhibitors, tissue inhibitors of metalloproteinases (TIMP). Inhibiting the collagenolytic pathway can enhance collagen accumulation. Addition of the NO donor SNAP to rat mesangial cells increases gelatinase A activity [52] whereas rat fibroblasts collagenase activity is unaffected by SNAP [41]. Another potential mechanism of posttranslational collagen regulation by NO is regulation of protein kinase C (PKC) activity [53 and 54]. By inhibiting PKC activity, NO could down-regulate PKC-related collagen synthesis in fibroblasts.

    when humans and animals are fed an arginine-enriched diet there is improved healing as measured by collagen deposition and breaking strength"



    "We also review the evidence that in certain nutritional or disease states or in certain stages of development otherwise dispensable amino acids may become indispensable. Arginine, citrulline, ornithine, cysteine, and tyrosine thus may be considered as acquired indispensable amino acids. Evidence for the indispensability of taurine is also considered. We propose a classification of the indispensability of amino acids based on clinical and therapeutic considerations."

    - Newer concepts of the indispensable amino acids. Laidlaw SA, Kopple JD. Am J Clin Nutr. 1987 Oct;46(4):593-605.

    "Some stress conditions that put an increased demand on the body for the synthesis of L-arginine include trauma (including surgical trauma), sepsis and burns. Under these conditions, L-arginine becomes essential, and it is then very important to ensure adequate dietary intake of the amino acid to meet the increased physiological demands created by these situations.

    L-arginine, even when it is not an essential amino acid as defined above, is a vital one. In addition to participating in protein synthesis, it plays a number of other roles in the body. These include the detoxification of ammonia formed during the nitrogen catabolism of amino acids via the formation of urea. In addition, L-arginine is a precursor in the formation of nitric oxide, creatine, polyamines, L-glutamate, L-proline, agmatin (a possible neurotransmitter in the brain) and the arginine-containing tetrapeptide tuftsin, believed to be an immunomodulator. L-arginine is a glycogenic amino acid; it can be converted to D-glucose and glycogen if needed by the body or it can be catabolized to produce biological energy.

    L-arginine, when administered in high doses, stimulates pituitary release of growth hormone and prolactin and pancreatic release of glucagon and insulin. Intravenous L-arginine may be used as an aid in the evaluation of problems with growth and stature that may be due to growth hormone deficiency. Intravenous arginine hydrochloride may be used as a fourth-line agent in the treatment of severe metabolic alkalosis. L-arginine is also used as an immunonutrient in enteral and parenteral nutrition to help improve the immune status in those suffering from sepsis, burns and trauma."

    "L-arginine supplements theoretically may potentiate the effects of organic nitrates if taken concomitantly."
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    C6H13NO2 pu12en12g's Avatar
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    Originally posted by pu12en12g
    L-arginine, when administered in high doses, stimulates pituitary release of growth hormone and prolactin and pancreatic release of glucagon and insulin.
    More on Prolactin:

    Prolactin is a hormone produced by the anterior pituitary gland in both men and women. It is known as a gonadotrophic hormone as it affects the gonads (testes and ovaries). It also has an effect on other organs in the body. However, only the effects on the reproductive organs will be discussed here.

    In males, prolactin influences the production of testosterone and affects sperm production. In conditions where prolactin secretion is increased (hyperprolactinaemia), testosterone levels drop and sperm production is reduced or absent, resulting in male infertility. <----- Very interesting. Anyone have any thoughts on this ?

    The main action of prolactin in females is the induction and maintenance of lactation (breastfeeding). Prolactin levels build up during pregnancy but milk secretion does not begin until after birth. As an infant suckles, prolactin is released into the mother's blood stream, causing the milk glands to produce more milk. Prolactin and other hormones are responsible for the development of mammary glands during pregnancy. Prolactin also affects the ovaries. The main target area is the corpus luteum, the secretory organ formed from the ruptured ovarian follicle after ovulation. High prolactin levels lead to reduced progesterone function. The result of hyperprolactinaemia (excess production of prolactin) can be the non-appearance of menarche (beginning of menstruation at puberty), amenorrhoea (absence of menstruation in a woman after puberty) and anovulatory menstrual cycles (absence of ovulation i.e. no mature eggs produced). These effects can be the basis of female infertility.

    There are many causes of increased prolactin secretion.
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    Originally posted by pu12en12g

    In males, prolactin influences the production of testosterone and affects sperm production. In conditions where prolactin secretion is increased (hyperprolactinaemia), testosterone levels drop and sperm production is reduced or absent, resulting in male infertility. <----- Very interesting. Anyone have any thoughts on this ?

    well ****, who wants my Cold Fusion and Nitrous? haha...
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    C6H13NO2 pu12en12g's Avatar
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    Effects of arginine and ornithine on strength, lean body mass and urinary hydroxyproline in adult males.

    Elam RP, Hardin DH, Sutton RA, Hagen L.

    "Twenty-two adult males participated in a 5 week progressive strength training program. One half the subjects received the amino acids L-arginine and L-ornithine and the other half, a placebo. The study used a double blind protocol so that subjects as well as investigators had no knowledge of which substances were being administered. Dosages amounted to 2 grams or 1 gram each of L-arginine and L-ornithine, and 600 mg of calcium and 1 gram of Vitamin C as placebos. These supplements were taken orally for a total of 25 administrations. Following the short term strength program using progressively high intensities, tests were taken for total strength (TS), lean body mass (LBM) and urinary hydroxyproline (UH).

    The results from ANOVA showed that subjects who were taking the arginine-ornithine combination scored significantly higher in TS and LBM (p less than .05), and significantly lower in UH (p less than .05), than subjects on placebos.

    It was concluded that arginine and ornithine taken in prescribed doses can, in conjunction with a high intensity strength training program, increase TS (Total Strength) and LBM (Lean Body Mass) in a relatively short period of time. Arginine and ornithine also aid in recovery from chronic stress by quelling tissue breakdown as evidenced by lower UH levels."
    Last edited by pu12en12g; 07-28-2004 at 06:43 AM.
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    Super Member CuttingEdge's Avatar
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    Just buy some arginine powder.

    I can't believe how much money people waste on NO products.
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    Originally posted by CuttingEdge
    Just buy some arginine powder.

    I can't believe how much money people waste on NO products.
    now i say **** arginine, just eat foods high in it like eggs, nuts, etc. Also your regular protein and protein shakes
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  20. #20
    Registered User Immichaelski's Avatar
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    Thats the main ingridient in any of these muscle pump supplements. It also causes herpes breakouts. Be careful
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    C6H13NO2 pu12en12g's Avatar
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    The Nitric Oxide + Creatine stack:

    The more muscle you gain, the greater the demand on creatine-synthesizing pathways to supply extra creatine to the newly added tissue. Arginine is required for the synthesis of creatine. Almost 2.5 grams of arginine are required to match the daily loss of creatine (as creatinine) in a 70-kg (~154-lb) male. By combining arginine with creatine, more arginine is free to promote nitrogen retention (essential for building muscle) and enhance sodium and water excretion (so-called 'muscle hardening' effects).

    "[O]rally administered arginine hydrochloride certainly has anabolic effects by increasing insulin secretion. This molecule has been often used for its anti-asthenic effects in convalescent patients. Weight gain often occurs in patients receiving this therapy. This weight gain is often considered to be a side effect but is to be taking into account when arginine is consumed."

    - Arginine researcher Dr. Adrien Schaefer
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    I have tried NO2 and NOx, and they did not work at all other than give me an accelerated heart rate, and made me horny the first couple of times I took it.
    As far as improving endurance and anaerobic performance it hurt more than helped. It was worse for mental performance making me jittery and unfocused.
    The only thing it was good for (marginally) was sexual arousal, but that was only the first couple of times using the stuff. After that, it hindered my performance.

    In short: a total waste of money.
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  23. #23
    C6H13NO2 pu12en12g's Avatar
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    Originally posted by polar-b
    I have tried NO2 and NOx, and they did not work at all other than give me an accelerated heart rate, and made me horny the first couple of times I took it.
    As far as improving endurance and anaerobic performance it hurt more than helped. It was worse for mental performance making me jittery and unfocused.
    The only thing it was good for (marginally) was sexual arousal, but that was only the first couple of times using the stuff. After that, it hindered my performance.

    In short: a total waste of money.
    Sounds like your diet was not up to par.
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    Banned rafael's Avatar
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    whenever any of you decides to post some article PLESE type the reference of the actual scientific research backing it up.

    or don't make any claims.

    by the way there is no reserch on no2

    "The active ingredient in NO2 that is supposed to produce these effects is arginine alpha-ketogluterate. Once the marketing smoke screen is swept aside, youll find there is no scientific evidence that even remotely substantiates these claims. None, zero, nada, zilch."Paul cribb.
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    C6H13NO2 pu12en12g's Avatar
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    Originally posted by rafael
    whenever any of you decides to post some article PLESE type the reference of the actual scientific research backing it up.

    or don't make any claims.

    by the way there is no reserch on no2

    "The ?active? ingredient in NO2 that is supposed to produce these effects is arginine alpha-ketogluterate. Once the marketing smoke screen is swept aside, you?ll find there is no scientific evidence that even remotely substantiates these claims. None, zero, nada, zilch."Paul cribb.
    Wow... could you possibly BE any MORE clueless ?
    Last edited by pu12en12g; 08-22-2004 at 10:40 AM.
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    Banned rafael's Avatar
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    Originally posted by pu12en12g
    Wow... could you possibly BE any MORE clueless ?


    you know what, never mind.

    go knock your self out with no2
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  27. #27
    i gotta stay fly-Y-yY-yyy zuche's Avatar
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    no2 is the best supplement out
    make sure to get ed byrds its allready a classic.

    the other brands arent as reliable
    i think muscletech also tried making an no2 tab

    if anyone has taken they should list results
    im kind of curious about it
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  28. #28
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    Originally posted by zuche
    no2 is the best supplement out
    make sure to get ed byrds its allready a classic.

    the other brands arent as reliable
    i think muscletech also tried making an no2 tab

    if anyone has taken they should list results
    im kind of curious about it
    NO2 is not the best bro, some ppl think so, but i have tried 3 bottles of it and it doesnt even compare to Syntrax's Nitrous, but then again results vary by user
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  29. #29
    i gotta stay fly-Y-yY-yyy zuche's Avatar
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    dude i had a good per pump but my blood flow wasn't working down there

    did you have any of these issues?
    why did you like syntrax's version better?

    and what is the price on it
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  30. #30
    i gotta stay fly-Y-yY-yyy zuche's Avatar
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    dude i had a good per pump but my blood flow wasn't working down there

    did you have any of these issues?
    why did you like syntrax's version better?

    and what is the price on it
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