Does piperine negate caffeine's stimuation?

[eatyourspinach]

*Stimulation

Effect of Black Pepper (Piper nigrum) Extract on Caffeine-Induced Sleep Disruption and Excitation in Mice

https://mdpi-res.com/d_attachment/nu...ion=1653967600

Abstract: Sleep is one of the most essential factors required to maintain good health. However, the global prevalence of insomnia is increasing, and caffeine intake is a major trigger. The objective of this study was to investigate the inhibitory effect of black pepper, Piper nigrum extract (PE), on caffeine-induced sleep disruption and excitation in mice. Caffeine significantly decreased sleep duration in the pentobarbital-induced sleep test. It also resulted in a significant increase in sleep onset and a decrease in non-rapid eye movement sleep. Moreover, in an open-field test, caffeine-treated mice exhibited a significantly increased time in the center zone and total distance traveled. However, the co-administration of caffeine and PE did not result in similar arousal activities. Thus, our results suggest that PE can be used as a potential therapeutic agent to treat sleep problems and excitatory status associated with caffeine intake

"Piperine, a major alkaloid isolated from black pepper,increases pentobarbitone sleeping time and has been identified as a positive allosteric modulator of γ-aminobutyric acid type A (GABAA) benzodiazepine receptor in in vitro cell models "

"We investigated whether PE could attenuate sleep disturbance induced by caffeine. In this study, caffeine (50 mg/kg) significantly (p <0.05) inhibited pentobarbital-induced sleep in ICR mice. Co-administration of PE at 100, 250, or 500 mg/kg with caffeine significantly (p <0.01) increased sleep duration compared to treatment with caffeine alone. Thus, PE can attenuate the wake-enhancing effects of caffeine"

[TheFugitive]

Nice

[air2fakie]

Those mice should stop drinking coffee hours before bedtime.