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  1. #1
    Registered User AlaaEmad's Avatar
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    Hydrolyzed protein

    I had bought a Hydrolyzed protein with chocolate flavor and I was wondering whether it's performance will be decreased if I mixed it with hot water instead of cold water ?
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    Registered User FormulaLT1poweR's Avatar
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    No, some people prefer to mix their whey protein with a hot beverage...completely up to you!
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    Registered User Misctake7's Avatar
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    Protein is just powdered food. Mix with whatever you want and it will be just fine.
    Progressive overload + progressive eating = gains. Simple as that!
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    WE LIVE THIS largenj's Avatar
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    either or bud, i know people who mix theirs with coffee to give them some flavor. Should be fine
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    Registered User changemyoil66's Avatar
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    Doesn't matter if you use hot or cold water, you still wasted money. Stick with a concentrate/isolate combo.
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    Originally Posted by changemyoil66 View Post
    Doesn't matter if you use hot or cold water, you still wasted money. Stick with a concentrate/isolate combo.
    Pretty much. Just pay attention to how the whey protein was processed.
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    Registered User kissdadookie's Avatar
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    Cold. Heating the protein makes it lose the micro fractions and I think the peptide bonds may degrade (the reason you use hydrolysate is for the quicker absorption for post or pre workout use, along with the di and tri peptides).
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    Cybergenics...it's bomb! lucia316's Avatar
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    Originally Posted by changemyoil66 View Post
    Doesn't matter if you use hot or cold water, you still wasted money. Stick with a concentrate/isolate combo.
    This

    Save your money next time.
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    evidence based poster Terrimonas's Avatar
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    Originally Posted by kissdadookie View Post
    Cold. Heating the protein makes it lose the micro fractions and I think the peptide bonds may degrade (the reason you use hydrolysate is for the quicker absorption for post or pre workout use, along with the di and tri peptides).
    And just passing through the acidic stomach denatures the protein also. And a hydrolyzed protein has already been denatured and indiscriminately chopped up so worrying about any of that is retarded.
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    Registered User kissdadookie's Avatar
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    Originally Posted by Terrimonas View Post
    And just passing through the acidic stomach denatures the protein also. And a hydrolyzed protein has already been denatured and indiscriminately chopped up so worrying about any of that is retarded.
    If you look at the absorption of di and tri peptides, they appear to get absorbed intact. So destroying those peptide bonds defeats the purpose of using a hydrolysate.

    Also, they've done a comparison study between freeform aminos vs intact proteins and the intact proteins were superior for gains (they did use rats I believe, but it was amino acid matched). So it does not appear that proteins simply get broken down to freeform aminos and thus one is interchangeable with the other.
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  11. #11
    22nd Street Barbell Marshall28's Avatar
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    shouldn't cause any issues.
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    Registered User kissdadookie's Avatar
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    Originally Posted by Marshall28 View Post
    shouldn't cause any issues.
    Defeats the purpose of using hydrolyzed protein.

    If all one cares about is the amino acid content, then yes, it wouldn't matter. However, if one already bought it, paid a premium for it, makes no sense to essentially destroy the one major property it has.
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  13. #13
    Cybergenics...it's bomb! lucia316's Avatar
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    Originally Posted by kissdadookie View Post
    Defeats the purpose of using hydrolyzed protein.

    If all one cares about is the amino acid content, then yes, it wouldn't matter. However, if one already bought it, paid a premium for it, makes no sense to essentially destroy the one major property it has.
    Perhaps, but OP should have just bought WPC, a blend, casein, whatever was less expensive and saved the money. Buying "premium" stuff that isn't doing anything better for you than the mentioned options means that, most likely, marketing came into play.
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    Originally Posted by lucia316 View Post
    Perhaps, but OP should have just bought WPC, a blend, casein, whatever was less expensive and saved the money. Buying "premium" stuff that isn't doing anything better for you than the mentioned options means that, most likely, marketing came into play.
    Not trying to tell the OP or anybody to keep buying whey hydrolysate, but if one already bought it, why essentially destroy it by mixing it in hot water? Now you're REALLY wasting your money because you've just destroyed the unique properties of the protein which you paid a premium for.

    So seeing a bunch of people say "yeah, mix it in hot water, it'll be fine" makes me scratch my head because that's essentially telling the OP "hey, you know that extra money you paid for it? now go mix it in hot water and nullify the qualities it has which you paid extra for."

    I mean, if someone has a tub of pure whey hydrolysate, I wouldn't even use it for any other purpose than pre, possibly intra, and post.
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    Originally Posted by kissdadookie View Post
    Not trying to tell the OP or anybody to keep buying whey hydrolysate, but if one already bought it, why essentially destroy it by mixing it in hot water? Now you're REALLY wasting your money because you've just destroyed the unique properties of the protein which you paid a premium for.

    So seeing a bunch of people say "yeah, mix it in hot water, it'll be fine" makes me scratch my head because that's essentially telling the OP "hey, you know that extra money you paid for it? now go mix it in hot water and nullify the qualities it has which you paid extra for."

    I mean, if someone has a tub of pure whey hydrolysate, I wouldn't even use it for any other purpose than pre, possibly intra, and post.
    OP is just talking about hot water. Doesn't protein need extreme heat to be denatured? Even hydrolyzed? Highly doubt the hot water OP is talking about would be warm enough to denature the protein.
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  16. #16
    Registered User kissdadookie's Avatar
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    Originally Posted by Misctake7 View Post
    OP is just talking about hot water. Doesn't protein need extreme heat to be denatured? Even hydrolyzed? Highly doubt the hot water OP is talking about would be warm enough to denature the protein.
    I think heat may destroy some of the peptide bonds and fractions of the protein. The amino acids really wouldn't be affected.

    Look at the different ways isolate is made, the low temp processing retains A LOT more of the fractions.

    Wouldn't really affect your macros, but hydrolysates should really be used for pre, possibly intra, and post anyway. Kind of a waste at other times since other times you can and should just use cheaper proteins ("benefit" of hydrolysates is mainly for the di and tri peptides, they are absorbed intact and doesn't really need digesting, thus suitable for pre, possibly intra, and post as it shouldn't bloat and should get taken up quickly similar to freeform aminos but with the additional benefit of being able to take in multiple aminos together at once due to the di and tri peptides).
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    Cybergenics...it's bomb! lucia316's Avatar
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    Originally Posted by kissdadookie View Post
    Not trying to tell the OP or anybody to keep buying whey hydrolysate, but if one already bought it, why essentially destroy it by mixing it in hot water? Now you're REALLY wasting your money because you've just destroyed the unique properties of the protein which you paid a premium for.

    So seeing a bunch of people say "yeah, mix it in hot water, it'll be fine" makes me scratch my head because that's essentially telling the OP "hey, you know that extra money you paid for it? now go mix it in hot water and nullify the qualities it has which you paid extra for."

    I mean, if someone has a tub of pure whey hydrolysate, I wouldn't even use it for any other purpose than pre, possibly intra, and post.
    I know, but I don't think hot water that's no where near boiling point is going to denature to a point where it's going to cause a complete loss of "goodness."

    If they have a tub, they should be educated for next time, but in reality. It's just protein and worrying about the use time period (I know it absorbs quickly, but that's really moot in the grand scheme of things) is what causes people to buy stuff like hydrolysate in the first place.

    I'm not convinced though that some hot water in the 120-140 degree range is going to cause the sky to fall.

    Note: I'm not disagreeing with your point about best time to use something like this, but stating for the sake of OP that it doesn't really matter in context with total diet.
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    Originally Posted by lucia316 View Post
    I know, but I don't think hot water that's no where near boiling point is going to denature to a point where it's going to cause a complete loss of "goodness."

    If they have a tub, they should be educated for next time, but in reality. It's just protein and worrying about the use time period (I know it absorbs quickly, but that's really moot in the grand scheme of things) is what causes people to buy stuff like hydrolysate in the first place.

    I'm not convinced though that some hot water in the 120-140 degree range is going to cause the sky to fall.

    Note: I'm not disagreeing with your point about best time to use something like this, but stating for the sake of OP that it doesn't really matter in context with total diet.
    Agreed, which is also why I think it's a waste to use the stuff at any other time apart from pre, possibly intra, and immediately post.
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  19. #19
    evidence based poster Terrimonas's Avatar
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    Originally Posted by kissdadookie View Post
    If you look at the absorption of di and tri peptides, they appear to get absorbed intact. So destroying those peptide bonds defeats the purpose of using a hydrolysate.

    Also, they've done a comparison study between freeform aminos vs intact proteins and the intact proteins were superior for gains (they did use rats I believe, but it was amino acid matched). So it does not appear that proteins simply get broken down to freeform aminos and thus one is interchangeable with the other.
    You really have no idea what you are talking about do you?

    First of all, slight heating WILL NOT break peptide bonds. It will denature the protein disrupting the secondary and tertiary structure. This is NOT the same a hydrolyzing a protein i.e. breaking peptide bonds.

    Secondly, hydrolysis is done primarily with endopepsidases leaving peptide chains of 2-3+ amino acids in length. Any important fraction has been chopped up for the most part.

    Thirdly, a hydrolsate by its very nature has had its peptide bonds "destroyed". The alleged "benefits" are because its peptide bonds HAVE BEEN destroyed!
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    Originally Posted by Terrimonas View Post
    You really have no idea what you are talking about do you?

    First of all, slight heating WILL NOT break peptide bonds. It will denature the protein disrupting the secondary and tertiary structure. This is NOT the same a hydrolyzing a protein i.e. breaking peptide bonds.

    Secondly, hydrolysis is done primarily with endopepsidases leaving peptide chains of 2-3+ amino acids in length. Any important fraction has been chopped up for the most part.

    Thirdly, a hydrolsate by its very nature has had its peptide bonds "destroyed". The alleged "benefits" are because its peptide bonds HAVE BEEN destroyed!
    Explain casein hydrolysate/peptopro and why it has a high amount of di and tri peptides (petide bonds still intact). Not contesting you, literally asking a question :P

    You could be right on the fractions though (I did not look into it that deeply so what I've posted are not intended to be definitive obviously).
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