Since I've been active on these forums, I've seen discussion after discussion on Taurine/Glycine padding. I decided to do some research on Kjeldahl Method for protein analysis, and after crunching some numbers have come up with what I believe to be some real world numbers, not just speculation. In this thread, I am going to reveal just how badly taurine/glycine can pad the protein content.
*Real Numbers Below*
First off, the Kjeldahl Method simply measures nitrogen content in a sample. That's all. Food typically consists of fat, carbohydrates, and protein, and since protein is the only one of those three that contain any nitrogen, the amount of nitrogen found in the sample is a good indicator of the protein content in a food sample.
The test measures total nitrogen content by weight and multiplies it by a certain factor. In dairy products, protein is measured by taking the weight of nitrogen found in the sample and multiplying it by a factor of 6.38. This is because in dairy products, the average amount of nitrogen found in protein (by weight) is ~15.67%. For example if a test found that 5g of nitrogen was present in a sample, they would multiply that by 6.38 to determine the protein content to be 31.9g.
Recall: The multiplying factor of 6.38 is based on the fact that the average amount of nitrogen found in dairy protein (by weight) is ~15.67%. To get 6.38, simply divide 1/0.1567 to get 6.38
Now for the good stuff:
To determine how badly taurine and glycine will pad the protein content, we must determine the percent nitrogen of each compound by mass. If you flunked chemistry, you'll just have to trust my calculations.
Glycine:
Glycine has a chemical formula of C2H5NO2 and has a molecular weight 75.0666g/mol.
Number of moles in 1g of glycine is:
1g / 75.0666g/mol = 0.0133215 mol
Since there is only 1 nitrogen in the chemical formula of glycine, we know that in 0.0133215 mol of glycine, there is 0.0133215 mol of nitrogen. To calculate the mass of nitrogen, multiply the moles of nitrogen by the molar mass of nitrogen (14.007 g/mol):
(0.0133215 mol N)(14.007 g/mol) = 0.18659425 g nitrogen.
To calculate the percent mass, we divide the amount of nitrogen by the total amount of glycine and multiply by 100%
0.18659425g / 1g x 100% = 18.66%
Glycine is 18.66% nitrogen by mass.
To determine how glycine would read on a protein scale, divide 18.66% by the average % of protein:
18.66% / 15.67% = 1.19
This means that for every gram of glycine present, 1.19g of protein will register.
Taurine:
Chemical formula: C2H7NO3S
Molecular weight: 125.15 g/mol
moles of Taurine in a 1g sample = 0.00799041 mol
0.00799041 mol taurine = 0.00799041 mol of nitrogen
mass of nitrogen in 1g taurine sample = 0.11192169g
% nitrogen in taurine by mass = 11.19%
11.19% / 15.67% = 0.714
This means for every gram of taurine present, 0.714g of protein will register.
So What Does This Mean?
According to the French label of a popular protein that contains taurine/glycine, there contains 1900 mg of glycine, and 2900 mg of taurine. This means that for:
Glycine: 1900mg x 1.19 = 2261 mg of protein registered
Taurine: 2900mg x 0.714 = 2070 mg of protein registered
This particular protein actually contains 4331mg (or 4.331g) of added amino acid protein. To calculate the true protein content, simply subtract 4.331g from the total.
***
Just for fun, I've done the calculations for creatine and betaine as well.
1 gram of Glycine registers as 1.19g of protein
1 gram of Taurine registers as 0.714g of protein
1 gram of Creatine registers as 2.05g of protein
1 gram of Betaine registers as 0.763g of protein
***
Hopefully we can now put this discussion to bed.
|
Closed Thread
Results 1 to 30 of 132
-
02-25-2014, 10:46 AM #1
Taurine/Glycine - The Discussion Ends. WARNING! Science Content Inside.
Last edited by mentalist72; 02-25-2014 at 11:07 AM.
-
02-25-2014, 10:51 AM #2
- Join Date: Mar 2011
- Location: Clifton, New Jersey, United States
- Posts: 23,002
- Rep Power: 243656
Science! But it doesn't change the fact that it still "pads" it to some extent which will always be a heated topic as some companies obviously are guilty of it.
My secret? Texting between sets.
-
02-25-2014, 10:51 AM #3
This might not end well
1. Brady's balls were perfect crew
2.
3.
4. Mopar Manlet Crew
-
02-25-2014, 11:03 AM #4
Very interesting post, reps on recharge!
BC, Canada
✧☆ COUNTRYMIKE Appreciation Crew ☆✧
Instagram: @KyleBJanzen
YouTube: https://goo.gl/VqHdWe
-
-
02-25-2014, 11:06 AM #5
You mean companies aren't trying to give customers more bang for their buck by including creatine?!?!?!?
Seriously, if all of your data is valid, this is very informative.
You used "how" instead of "now" on at least two occasions though.Staples:
The Father
The Son
The Holy Spirit
-
02-25-2014, 11:06 AM #6
- Join Date: May 2011
- Location: Coalinga, California, United States
- Age: 33
- Posts: 48,213
- Rep Power: 451500
INB4 supp companies quote this as reasoning to pad the **** out of protein. Interesting read though.
Short cuts to success are often paved with lies.
1/13/16: Massive hernia.
5/10/16: Finally back to lifting, light but improving.
Why Teens shouldn't cut/Lack of progress thread- http://forum.bodybuilding.com/showthread.php?t=169272763&p=1397509823#post1397509823
-
02-25-2014, 11:08 AM #7
Haha, thanks for the heads up. Fixed it
My data is based on a conversion factor of 6.38 which is normally used on milk products. Since protein powder (whey, casein) is made from milk, that's the conversion factor I assume they use. A smaller conversion factor would lower the overall protein content.Last edited by mentalist72; 02-25-2014 at 11:35 AM.
-
02-25-2014, 11:08 AM #8
- Join Date: Sep 2011
- Location: Maine, United States
- Posts: 14,610
- Rep Power: 113248
You should run the numbers on Melamine, then realize how much scarier spiking could get.
-
-
02-25-2014, 11:09 AM #9
- Join Date: Nov 2009
- Location: Provo, Utah, United States
- Age: 29
- Posts: 1,659
- Rep Power: 1110
Strong. Thanks man for the education
-
02-25-2014, 11:09 AM #10
-
02-25-2014, 11:11 AM #11
-
02-25-2014, 11:24 AM #12
- Join Date: Sep 2011
- Location: Maine, United States
- Posts: 14,610
- Rep Power: 113248
Gets worse.
Complemented with Cyanuric Acid, it forms a plasticy compound. Which is fine when it's outside the body. But when you're an infant consuming melamine spiked formula, or a dog/cat consuming melamine spiked pet food, those compounds can form inside your kidneys to cause kidney stones.
-
-
02-25-2014, 11:24 AM #13
-
02-25-2014, 11:33 AM #14
- Join Date: Feb 2007
- Location: the best h20, comes from, Fiji
- Posts: 45,692
- Rep Power: 486578
Nice work OP
It's hard to win an argument with a smart person. It's damn near impossible to win an argument with a stupid person. - Bill Murray
Mods - my avatar is locked by the admin and can't be changed.
-
02-25-2014, 11:34 AM #15
-
02-25-2014, 11:39 AM #16
-
-
02-25-2014, 11:43 AM #17
- Join Date: Nov 2010
- Location: Chugiak, Alaska, United States
- Posts: 610
- Rep Power: 5472
So basically Taurine and Glycine isn't as bad as people make it out to be but Creatine is worse? And yes I did flunk out on chemistry
-
02-25-2014, 11:46 AM #18
- Join Date: Sep 2007
- Location: Florida, United States
- Age: 51
- Posts: 22,582
- Rep Power: 91685
I had to ninja delete. I just realized it advertised a competitor... I will try and find another.
-
02-25-2014, 11:49 AM #19
thanks OP - will rep on r/c
100% 中國人
-
02-25-2014, 11:53 AM #20
-
-
02-25-2014, 11:55 AM #21
- Join Date: Aug 2011
- Location: New Jersey, United States
- Age: 35
- Posts: 8,523
- Rep Power: 8384
Cliffs:
Don't buy french protein
or did I miss something?
-
02-25-2014, 11:57 AM #22
Good information, OP. Thanks for putting all of that together...I think.
Greens on recharge.
-
02-25-2014, 11:58 AM #23
Thanks for the great post OP, Very informative Repped!
♛♛♛♛ ♛DL- 405 Bench- 275 Squat- 365♛♛♛♛
*Always looking for free samples Pm if you have any* :)
★Accept the pain of discipline or Accept the pain of regret★
➲Goals: DL: 485 Squat: 415 Bench:305
Bench:275 Reached on 2/24/2014
-
02-25-2014, 12:00 PM #24
- Join Date: Sep 2007
- Location: Florida, United States
- Age: 51
- Posts: 22,582
- Rep Power: 91685
Yeah you did. Seems that a french label of a popular protein powder includes the specific amount of taurine and glycine included per serving. The USA label does not provide the consumer with this information. When you extrapolate the amount of these added aminos from the per serving label claims of "protein" you see that the actual "protein" is lower. We can now get a much better idea as to how much lower.
Sadly, the labeling regs seem to permit this due to using nitrogen as a determiner of protein content, but we don't have to sit back. We can demand transparency. Many people seemingly won't care, but shouldn't the consumer have the info to be able to make the decision?
-
-
02-25-2014, 12:10 PM #25
What do you think about, let's say, companies that list on the ingredient panel "Servings size: 1 scoop (30g)" when there is no possible way to get 30g of powder into a scoop that size provided. Rather, 30g would be one giant heaping scoop? Even after settling and shaking the scoop when it's full - there is no physcial way to get 30g into 1 scooper.
Do you think this is label dishonesty?
-
02-25-2014, 12:11 PM #26
-
02-25-2014, 12:12 PM #27
- Join Date: Sep 2007
- Location: Florida, United States
- Age: 51
- Posts: 22,582
- Rep Power: 91685
Good question, but if they said 30 grams, then it wouldn't be dishonest. Everyone should use a digital scale at least once when they start a supplement IMO. That is how I saw issues with a specific flavor of a popular BCAA product. And I took a pic and posted it on this forum, and a rep even made a video response (using a different flavor). I am an equal opportunity kind of guy. Any brand is fair game. I grabbed one that everyone is talking about and one where we have a good idea of the numbers. If someone offered data on another brand, those images would be generated as well.
-
02-25-2014, 12:15 PM #28
- Join Date: Feb 2013
- Location: New York, New York, United States
- Age: 43
- Posts: 16,991
- Rep Power: 91765
Completed Logs & Reviews:
Clear Muscle Log: http://forum.bodybuilding.com/showthread.php?t=161906833&p=1244983053#post1244983053
"Now that you've got a basic degree of strength you can now proceed with success onto a bodybuilding type program - which I am not experienced in providing. You've exceeded what I focus on, and that is beginner strength gains." - Mark Rippetoe
-
-
02-25-2014, 12:16 PM #29
-
02-25-2014, 12:17 PM #30
I agree with you except for the fact that "supp consumers are generally intelligent people...". I'm not saying that aren't intelligent but rather that many people who buy protein powder probably don't know about protein padding. When I first starting lifting, the only thing I looked at on the label was the amount of protein. I can't be the only one.
In a way, yes, but in the end, you're still getting the full amount. Let say the label advertises 75 servings at a 30g scoop. If that scoop was only 25g you'd end up getting 90 scoops. It might mess up your macros but at least you're getting the full advertised weight.
Bookmarks