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  1. #1
    Tiptoe Through the Tulips eXistenceLies's Avatar
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    **Offical Melanotan 1 and 2 Thread**(Tons of info)

    This should settle all those Melanotan threads that keep popping up. Im going to give as much info I can from other sources. Everything will be from http://melanotan.org/cgi-bin/yabb/YaBB.pl and for more info http://articles.muscletalk.co.uk/article-melanotan.aspx. For actual responses from people go there, but here I will list as much info as I can find. If anything posted under this is against the rules. I will remove it due to Mods authority.

    *PRE-MIXED MELANOTAN PEPTIDES MAKE PEOPLE SICK*
    *DO NOT BUY MELANOTAN PEPTIDES ADULTERATED WITH A FILLER LIKE MANNITOL - DEMAND HIGH PURITY >99%*

    At no point in time will anything in Melanotan FAQs or forums override licensed medical practitioner to patient directives regarding matters of health. Direct doctor to patient counsels/advice ALWAYS takes precedence. Melanotan is not to be considered a legitimate source for direct medical advice. No individual is authorized to give direct medical advice on Melanotan. For direct medical matters always seek the assistance of licensed and qualified medical practitioners, NEVER rely upon Melanotan for such matters.

    These WARNING / FAQ posts have been formulated both from information found in published clinical trials of the melanotan peptides and also from anecdotal reports.

    Individuals considering using either of the as of yet unlicensed and unregulated melanotan peptides should know that there can be serious risks to one's health in doing so. Be aware of Governmental Health Agency Warnings specific to the melanotan peptides
    against their usage outside of regulated clinical trials. Before reading this FAQ please review the entirety of the first post of this thread.

    HEALTH CONCERNS - SEEK MEDICAL ASSISTANCE
    If a health concern should present itself during a regimen do not automatically assume it stems from some aspect of the regimen. Instead, do not hesitate to be medically examined by a licensed health care practitioner. Be as upfront with the practitioner about your regimen as possible and know that generally speaking the more information doctors have the better they will be in a position to help you.

    SEEING DERMATOLOGISTS
    Section summary: It is strongly recommended to have one's skin examined by a dermatologist to check for any skin issues prior to starting usage of a melanotan peptide. This is also true if any skin issue should become apparent while using a melanotan peptide or after finishing a regimen.

    Because usage of the melanotan peptides by people is a relatively new phenomenon (ie: the first human clinical trial of melanotan-1 was in 1991), it is advisable to visit a dermatologist to have a skin "check up" (physical) particularly for those who've never been able to tan previously. Request that the dermatologist do a thorough examination of your skin to check for any irregularities (ie: differences in skin tone, latent yeast/fungal infections, moles, etc.) Discuss with the dermatologist what are the aspects of skin tanning that individuals in general should be aware of in terms of how one's skin can be affected (besides becoming tan). Be aware that when developing a natural tan (natural - meaning based on melanin - and particularly for those who've never had a tan before) it is possible (for a relatively small population of individuals affected by various skin disorders) that certain areas of skin ("white spots"/patches) will not be able to tan and conversely certain areas may remain tanned ("brown spots"/patches) after completely ceasing a tanning regimen. There are many known causes (most of which are of an infectious <ie: yeast/fungal> nature) for these types of spots/patches and ordinarily this type of thing is not something that affected pale individuals in particular will become aware of until they can achieve a tan. If one is diagnosed with a dermal yeast infection (like tinea versicolor) they will generally be prescribed very effective and simple to use medicines (ie: Nizoral) for treatment. This is why it is a good idea to consult with a dermatologist to see if there are any issues that you may not be aware of. Additionally, you may want to see what information the dermatologist can share with you (if any) about the melanotan peptides. Such a checkup (ie: in the guise of a, "skin cancer screening") may be covered by your country's national healthcare system or your private/company healthcare plan in which case there will be either no charge for you or simply a small fee.

    MOLES & FRECKLES
    Section summary: Those with any moles (especially on the face) and/or freckles should know that they will almost certainly darken* and that additional not particularly visible moles will likely become more noticeable when tanning especially while using a melanotan peptide (initially), if such darkening will bother you then Melanotan.org strongly advises either not starting use of a melanotan peptide or having such moles and/or freckles removed and skin completely healed prior to starting a usage regimen.

    Those with a prevalence of moles and/or prominent freckles should be aware that any sort of tanning of one's skin can affect them and in particular when tanning with the usage of the melanotan peptides (darkening - particularly moles). Indeed, previously latent (not noticeable) moles can become visible / more visible when one starts to tan (regardless of using the peptides or not). Moles and freckles are concentration points for melanin and accordingly they will very likely be the first places to accumulate melanin prior to other areas of the skin (which for some people at least in the initial stages of using one of the peptides can set up a disagreeable contrast - particularly if not slowly building up a tan with lower than normal doses of peptide*). It may be advisable to undergo simple and safe mole and/or freckle removal treatments with a well qualified dermatologist prior to starting any melanin tanning regimen. A good example of the appearance enhancing benefits of having moles removed can be found in the following log where a forum member underwent Radio Surgitron and Intense pulsed light mole removal treatments in correspondence with a desire to have a natural looking tan. These types of treatments may be covered by your healthcare plan (best to inquire). If scarring is likely to occur, do not have moles removed or undergo other potentially scar causing procedures while using a melanotan peptide see the "SCAR FORMATION" section below.

    It is not currently definitively known how long it can take for melanotan peptide darkened moles to fade. The cosmetic ramifications of mole darkening and a progression of fading or not is an aspect of melanotan peptide usage about which no scientific studies are known to have been published.

    *: There has been some speculation in the forums that at certain dosing levels (at least initially) and progression speed of tanning moles can darken disproportionately fast relative to surrounding skin and this is what has caused issues for folks who've experienced this. Correspondingly with this speculation there is a debate that if a person builds up their tan slower than normal with lower than normal dosing that such a disproportionate contrast will not occur and instead a more natural and even tanning across the skin (including moles/freckles) will happen. Another view is that this initial difference in contrast is unavoidable.

    UV EXPOSURE
    Section summary: For fair skinned peptide users limited UV exposure is likely necessary and helps both darker skinned and fair skinned individuals have a more natural looking tan. Never overexpose oneself to UV light. UV light overexposure is known to cause skin damage (particularly cumulatively) that can lead to skin cancers. Get only enough UV exposure to effectively tan and not more (start with low levels for short amounts of time).

    While the administration of Melanotan peptides via injections has been clinically demonstrated to make it possible for people who ordinarily cannot tan or have difficulty doing so (type I/II individuals) to be able to as well as make it easier for type III and IVs to do so (even with no UV exposure) they will generally not be most effective and the resulting skin tones will not look most natural without some limited exposure to UV light (ie: sunlight).

    Real sunlight is the best, but sunbeds work too.
    The amount of UV exposure necessary to achieve good results differs for EVERY person.
    Some people will see their skin darken after only one sunbed session, while it will take a few sessions for others.

    Usage of melanotan-1 has been clinically demonstrated to reduce UV skin damage (not completely prevent it). While Melanotan II hasn't been clinically demonstrated to do this, because it allows one to tan in the same way, it is quite likely that this damage reduction effect is also found with its usage. Because UV light overexposure can negatively impact and damage skin it is best to limit exposure to a level that is just effective enough in producing a tanning effect but not more. Optimally (particularly when starting out) not more than 5 minutes in one session per day. Do not overexpose your skin to UV light, the melanotan peptides weren't designed to make skin capable of tolerating UV overexposure. Regardless of peptide usage, overexposure will damage your skin. Please know that UV skin damage is cumulative meaning that damage will build up over time. Cumulative damage has been shown to correlate with subsequent development of skin cancers and while scientists hypothesize that using the melanotan peptides can have a protective effect against skin cancers this has yet to be clinically demonstrated. Please note that melanin converts UV light into heat, as one becomes darker the skin will become noticeably warmer in the presence of the same tanning level of UV light as prior to being darker. This is a good sign and means that the increased level of melanin is providing additional photoprotection.

    In clinical trials on melanotan-1 it was reported that areas of the skin that have been more chronically exposed to UV light (ie: face, back of the neck, arms, and hands) were found to respond more readily to the melanogenesis (melanin producing) effects of the peptide. This effect has been reported in logs on the site as well (for melanotan II usage). For proper tanning balance it is advisable that early in getting UV exposure to develop a melanotan peptide tan one uses a strong SPF sunblock (30+ SPF) or towels to cover areas most subject to daily UV exposure (ie: the face, back of the neck) to allow other less chronically sun exposed areas to tan first and then subsequently expose the face (and other areas) for less time (or as needed) to UV light to thereby achieve a well balanced tan.

    When exposing oneself to UV light it is imperative to wear proper eye protection. Eyes are very sensitive to UV light and can be damaged irreparably from chronic and/or overexposure. If using sunbeds do not simply "scrunch up" your eyes during exposure but wear proper UV blocking protective eyewear (this is usually supplied by a salon). This cannot be emphasized enough.
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  2. #2
    Tiptoe Through the Tulips eXistenceLies's Avatar
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    TAN DURATION

    Section summary: Avoid any procedure and/or events that could lead to scarring in the weeks/months prior to or while using a melanotan peptide. Scars formed concurrently while skin undergoes changes in pigmentation (ie: tan formation) occur can become permanently discolored. Wait for any forming scars to completely heal and form and do not start or use a melanotan peptide during this period.

    The formation of relatively significant hypertrophic type scars (ie: keloids) are known to become discolored when the wound and the skin surrounding the wound concurrently undergo changes in pigmentation (ie: melanogenesis - tan formation). In an article on "scar revision" the United States' National Institutes of Health (NIH) counsels those who undergo such a procedure to, "Avoid exposure to the sun for several months after treatment. Use sunblock or a dressing (such as a Band-Aid) to keep the sun from permanently tanning the healing scar." in the recovery section of the page. The reason for this counsel is to prevent the scar from becoming impregnated with melanin stemming from the body's melanogenesis process. While actively using the melanotan peptides melanogenesis occurs regardless of UV exposure (as shown by the 1991 clinical study). What this means is that if one begins to form a scar while taking one of the melanotan peptides it is possible that the scar will become discolored relative to the rest of the skin surrounding it. When hypertrophic type scars are forming it is very advisable for cosmetic reasons to not use a melanotan peptide. Those planning on undergoing potentially scar forming procedures/surgery should not begin using a melanotan peptide and those using a peptide should stop doing so as much time in advance as possible. Once scar formation starts it is best to abide the NIH's advice and wait until a scar has completely healed and finished forming before undergoing any melanogenesis processes (don't use a melanotan peptide). Depending upon the severity of the scar(s) this can take up to several months.

    SCAR FORMATION COSMETIC ISSUES
    Section summary: Avoid any procedure and/or events that could lead to scarring in the weeks/months prior to or while using a melanotan peptide. Scars formed concurrently while skin undergoes changes in pigmentation (ie: tan formation) occur can become permanently discolored. Wait for any forming scars to completely heal and form and do not start or use a melanotan peptide during this period.

    The formation of relatively significant hypertrophic type scars (ie: keloids) are known to become discolored when the wound and the skin surrounding the wound concurrently undergo changes in pigmentation (ie: melanogenesis - tan formation). In an article on "scar revision" the United States' National Institutes of Health (NIH) counsels those who undergo such a procedure to, "Avoid exposure to the sun for several months after treatment. Use sunblock or a dressing (such as a Band-Aid) to keep the sun from permanently tanning the healing scar." in the recovery section of the page. The reason for this counsel is to prevent the scar from becoming impregnated with melanin stemming from the body's melanogenesis process. While actively using the melanotan peptides melanogenesis occurs regardless of UV exposure (as shown by the 1991 clinical study). What this means is that if one begins to form a scar while taking one of the melanotan peptides it is possible that the scar will become discolored relative to the rest of the skin surrounding it. When hypertrophic type scars are forming it is very advisable for cosmetic reasons to not use a melanotan peptide. Those planning on undergoing potentially scar forming procedures/surgery should not begin using a melanotan peptide and those using a peptide should stop doing so as much time in advance as possible. Once scar formation starts it is best to abide the NIH's advice and wait until a scar has completely healed and finished forming before undergoing any melanogenesis processes (don't use a melanotan peptide). Depending upon the severity of the scar(s) this can take up to several months.

    SOURCING A MELANOTAN PEPTIDE
    Section summary: Only use pharmaceutical grade high purity (99%+) melanotan peptides preferentially sold in vacuum sealed vials. Independent testing of what one purchases allows for the highest confidence that what one has bought is what it was sold as.

    As of 2010 the melanotan peptides are not approved for human usage by any governmental regulatory health agencies outside of human trials (for melanotan-1), as a result there are no government recognized/regulated sources for either melanotan peptide for human usage purposes. If one is going to experiment with a melanotan peptide it is imperative that one uses pharmaceutical grade (GMP) high purity peptide (99% and higher purity) preferentially sold in vacuum sealed vials. Never purchase or use peptides from a source that cannot guarantee that what they are selling is pharmaceutical grade (meaning fit for human usage) regardless of whether or not they are sold for human usage or not and preferably buy peptide sold in vacuum sealed vials. Avoid unprofessional sellers like the plague, this is your health/life on the line. There are very real health dangers of using peptides that are either made at a substandard purity level, contaminated / adulterated / putrified (gone bad), or something completely else (counterfeit) other than a melanotan peptide. Avoid sellers offering the peptides in "premix" form. Premixed melanotan peptides are going to be degraded by the time they are delivered. Such degraded peptide will cause allergies and/or general sickness (especially over time). It is unprofessional for a supplier to even offer premixed peptide and again, unprofessional suppliers should be avoided at all costs.

    For extra safety melanotan.org recommends that buyers have their peptides independently tested for purity. Such independent testing will allow for the highest degree of confidence that what one has purchased is indeed a high purity melanotan peptide. Avoid peptides that are sold with any fillers (ie: mannitol) added to them as such fillers can drastically alter the way the body will react to a given dosage of peptide. Be skeptical about any forum connected with a given seller, chances are the forum will not fully reflect an independent view of what people's experiences are with what that supplier is selling and/or the peptides in general.

    Why is purity so important?

    One of the primary reasons that purity is so important is that over time one can develop an allergy to non-melanotan peptide substances found in vials and subsequently not be able to use such vials having such impurities (even in very small quantities). This is also true for peptide that is not kept fresh. Over time peptides will begin to decay due to oxidation and/or natural bacterial processes if not stored properly (much like food left out or left for a long time in a refrigerator). This is why it is helpful to purchase peptides sold in vacuum sealed vials.

    Why are vacuum sealed vials preferred and how does one know if a vial is under vacuum?

    Vacuum sealed vials are preferred because the vacuum helps prevent oxidation and bacterial decay of the peptide itself. Without a seal oxidation will begin to make the peptide convert into something that is not a melanotan peptide and bacteria are able enter into a given vial (via air contamination) and cause a given vial of peptide to begin to spontaneously decay. Also, any miniscule quantities of bacteria that might have been introduced into a given vial during manufacturing have a much better chance to multiply in a non-vacuum sealed vial.

    When injecting bacteriostatic water into a vial the vacuum will draw the water into the vial slightly. If the water in the syringe is not drawn into the vial (after initial piercing) then it's a sure bet that the vial either was never vacuum sealed or lost it's vacuum.

    Where can I get peptides tested?

    A company called M-Scan Inc. has been used in the forums by members. Their website is found here:

    http://www.m-scan.com/

    DOSING AMOUNT GUIDELINES
    Find your weight on the chart below. 10mg is the average amount of one cycle. About 3 cycles are needed for the "loading phase." For someone of average weight (140-175), around 30mg is needed. This amount will vary from person to person. Heavier people, and skin type 1's need more than lighter people and those with higher skin types.

    -----
    10 mg / 12.00 injections = 0.833 mg --- 42 kg = 92 lbs @0.02mg/kg
    10 mg / 11.50 injections = 0.870 mg --- 43 kg = 96 lbs @0.02mg/kg
    10 mg / 11.00 injections = 0.909 mg --- 45 kg = 100 lbs @0.02mg/kg
    10 mg / 10.50 injections = 0.952 mg --- 48 kg = 105 lbs @0.02mg/kg
    10 mg / 10.00 injections = 1.000 mg --- 50 kg = 110 lbs @0.02mg/kg
    10 mg / 9.50 injections = 1.053 mg --- 53 kg = 116 lbs @0.02mg/kg
    10 mg / 9.00 injections = 1.111 mg --- 55 kg = 122 lbs @0.02mg/kg
    10 mg / 8.50 injections = 1.176 mg --- 59 kg = 130 lbs @0.02mg/kg
    10 mg / 8.00 injections = 1.250 mg --- 63 kg = 138 lbs @0.02mg/kg
    10 mg / 7.50 injections = 1.333 mg --- 67 kg = 147 lbs @0.02mg/kg
    10 mg / 7.00 injections = 1.429 mg --- 71 kg = 157 lbs @0.02mg/kg
    10 mg / 6.75 injections = 1.481 mg --- 74 kg = 163 lbs @0.02mg/kg
    10 mg / 6.50 injections = 1.538 mg --- 77 kg = 170 lbs @0.02mg/kg
    10 mg / 6.25 injections = 1.600 mg --- 80 kg = 176 lbs @0.02mg/kg
    10 mg / 6.00 injections = 1.667 mg --- 83 kg = 184 lbs @0.02mg/kg
    10 mg / 5.75 injections = 1.739 mg --- 87 kg = 192 lbs @0.02mg/kg
    10 mg / 5.50 injections = 1.818 mg --- 91 kg = 200 lbs @0.02mg/kg
    10 mg / 5.25 injections = 1.905 mg --- 95 kg = 210 lbs @0.02mg/kg
    10 mg / 5.00 injections = 2.000 mg --- 100 kg = 220 lbs @0.02mg/kg
    10 mg / 4.75 injections = 2.105 mg --- 105 kg = 232 lbs @0.02mg/kg
    10 mg / 4.50 injections = 2.222 mg --- 111 kg = 245 lbs @0.02mg/kg
    10 mg / 4.25 injections = 2.353 mg --- 118 kg = 259 lbs @0.02mg/kg
    10 mg / 4.00 injections = 2.500 mg --- 125 kg = 276 lbs @0.02mg/kg
    -------------

    For example, someone weighing 184 lbs should be taking a dose of 1.66667mg daily. They will be able to get this amount out of the vial 6 times ( 10 / 1.66667 = 6)

    Many people have began to emulate the slow-release method by giving themselves multiple injections daily. For example, that 184 lb. person would take 2 injections (1 morning / 1 night) of about .8333mg.


    SYRINGES (NEEDLES / PINS)
    Any insulin syringe will work. These are usually disposable and come in 10 packs. BD is a great brand. Buy something that is 28,29, or 30 guage. The higher the gauge, the smaller the opening at the end of the needle. That means the higher the gauge, the less pain incurred. 28,29,30 guage. 1/2 inch needle. 1cc, 1/2cc, or 1/3cc will work.

    Individuals planning on commencing a peptide regimen will need approximately 50 individually hermetically sealed/packaged, sterile syringes (DO NOT USE syringes that come unpackaged) for one month of usage with everyday dosing (30 for each day and ~ 20 for mixing purposes and as a margin for error).

    DO NOT REUSE DISPOSABLE SYRINGES, doing so is dangerous and can have serious health consequences (ie: bacterial infection and abscess formation).

    DO NOT SHARE SYRINGES, doing so is dangerous and will lead to the spread of infections (ie: blood borne viruses) found in any one of the individuals sharing a needle (ie: HIV-AIDS, Hepatitis C, etc.).

    DO NOT USE OUT OF DATE SYRINGES, syringes have an expiration date which is marked on the side of the box they are sold in. Use only in date (non-expired) syringes. Using out-of-date syringes will very likely make you sick.
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  3. #3
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    Reconstitution
    Melanotan peptides come in powder form. They cannot be injected in powder form, so they have to be mixed with a dilutant. The best dilutant is in date (not expired) bacteriostatic water. DO NOT USE water that is beyond its expiration date. Such water can be ordered typically inexpensively online. NEVER USE TAP WATER AS IT IS UNSAFE AND WILL LEAD TO BACTERIAL INFECTIONS OR OTHER COMPLICATIONS ARISING FROM OTHER CONTAMINANTS. Use only in date MEDICAL GRADE water sold specifically for injection purposes.

    When you reconstitute, take your syringe and stick it into the vial holding bac. water. Turn it upside down, and draw out the desired amount. This is usually between 1/2cc and 1cc.
    If you have 1/2cc syringes, draw out liquid up to the 50 mark on the syringe. This will fill up the whole syringe, since the markings only go up to 50.

    Now, look on the chart and find out how many injections you can get from the 10mg vial. From the above example, we are using a 184 lb. person. This person gets 6 injections from the 10mg vial. Therefore, draw out 50 units of bac water and shoot it into the vial. Repeat this 5 more times.

    6 injections ->
    1)Draw out 50 from bac. water, shoot into melanotan II vial
    2)Draw out 50 from bac. water, shoot into melanotan II vial
    3)Draw out 50 from bac. water, shoot into melanotan II vial
    4)Draw out 50 from bac. water, shoot into melanotan II vial
    5)Draw out 50 from bac. water, shoot into melanotan II vial
    6)Draw out 50 from bac. water, shoot into melanotan II vial

    You now have 300 units of bac. water in your melanotan II vial. Roll it on a hard surface for a while, until the melanotan II dissolves. (do not shake)

    If you are going with 1 injection per day, every time you are ready to inject, draw out 50 units.

    If you are going with 2 injections per day, every time you are going to inject, draw out 25 units.

    Here is what an image of 10 mg of melanotan II reconstituted with 1 cc of bacteriostatic water will look like:




    Note that the bacteriostatic aqueous solution is colorless and clear (not milky/cloudy).

    INJECTIONS
    See the Subcutaneous injection FAQ.
    The preferred method is a sub-cutaneous injection into the fat above the abdomen.
    If you are not familiar with how to inject, watch this video:
    Real player:
    http://www.pharmacy.unc.edu/carelabs...s/subqtape.ram

    AVI (windows media player):
    http://www.pharmacy.unc.edu/carelabs...s/subqtape.avi

    STORAGE
    Melanotan II in powder form - freezer
    Reconstituted melanotan II - refrigerator

    The melanotan II in the freezer will last for many months without degrading.

    The reconstituted melanotan II will begin to degrade around 2 weeks in the fridge.

    An alternative to keeping reconstituted vials in the fridge is preloading syringes. If you plan to get 6 injections out of your vial, then draw your dose into all 6 syringes at the same time. Place them in the freezer. When you are ready to inject, take one out, let it sit at room temp for 5 minutes (to thaw), then inject.


    http://peptide-guide.com/melanotan2.html

    http://peptidecalculator.com/calculator.html

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  4. #4
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    New Research on the Dangers of melanotan II Overdosing
    I know I have been missing in action for quite awhile, but I have some important information that I wanted to share with you all. People have asked me from time to time if there are any long-term or short-term dangers associated with taking Melanotan. I have always maintained that it is non-toxic to the human body as has been documented in the original Epitan Clincal Phase I/II trials. However, most of these safety trials were performed using the "melanotan-1" version of the drug, which is a linear polypeptide and only slightly different from the natural human a-MSH hormone. On the other hand, melanotan II is a more radical departure from the afore-mentioned polypeptide. This polypeptide is cyclic and it is this shape that enables it to bind more tightly to all of the melanocortin receptors than the linear version, which binds best to the melanocortin receptor (MC-1R) found on melanocyte cells.

    Because melanotan II is a foreign substance to the body, it invokes an immune system response akin to an allergic reaction. Many of the so-called side-effects you have either heard about or experienced yourself are due to an abundant overdose of melanotan II in your blood. Wheezing, sneezing, congestion, facial flushing, and nausea are all possible symptoms. But your immune system must first be taught to recognize the melanotan II peptide before you might actually experience these side-effects ... which is why first time users usually don't have any adverse reactions. Upon first introduction (or perhaps over several events), your immune system produces a quantity of antibodies that are capable of recognizing the melanotan II peptide. These antibodies attach to the surfaces of Mast cells in the tissue and Basophils in the bloodstream. When next they encounter the specific foreign substance to which they were designed to detect, they release powerful chemicals such as histamine and heparin into the bloodstream. And it is this release of chemicals which causes the side-effects of the drug.

    The degree to which the side-effects occur is directly proportional to how much peptide is in the bloodstream at one time. In the extreme case, anaphylactic shock is a possibility. This is a life-threatening condition characterized by swelling of body tissues, including the throat and hands, headache, uncontrollable itching, constriction of the airways and gastrointestinal symptoms such as abdominal pain, cramps, vomiting, and diarrhea, and a sudden drop in blood pressure. Since most of these dangers stem from the release of the amino acid derivative, histamine, by cells of the immune system, it is my suggestion that some form of anti-histamine be used as a preventive for those people who are especially sensitive to taking melanotan II. Just as well, much lower concentrations of the injected drug would also lessen the side-effects. For those taking notes, extrapolation of this principle out to its limits explains why a slow-release formulation of the drug is so invaluable and so necessary.

    There are several types of anti-histamine on the market today including Zyrtec, Allegra, Claritin, Clarinex, and Benadryl. But these drugs can be divided into 1st generation and 2nd generation types. Older drugs such as Benadryl are less selective to the Histamine (H1) receptor and thus, generally cause certain side-effects such as drowiness and/or dizziness and dry mouth. In fact, many over-the-counter sleep aids contain the same active ingredients as Benadryl. The newer 2nd generation anti-histamines are far more selective to H1 and have an improved tolerability profile. The anti-histamine thought to work best is Zyrtec and Allegra. Allegra is non-sedating while Zyrtec may cause some drowiness in certain individuals. Claritin and Clarinex are minimally sedative.

    In order to achieve the protection afforded by the anti-histamine, you will want to take it about 45-60 minutes before delivery of melanotan II. What you will achieve by doing this is a blocking of the Histamine receptors such that when Histamine is released into the bloodstream due to an allergen response, it has no where to attach and you will thus ward off an allergic reaction.


    This is from New England Peptide ...

    CUSTOM PEPTIDES

    Q: What do I do with the ends of my peptides, keep them free or block them?
    A: Peptides are created to mimic proteins. In order to mimic protein behavior, we must synthesize a peptide that has similar structure and charge. When a peptide is “cut out” from a protein, charges on the ends of the fragment peptide will be different than the parent. We have to alter synthesis strategies to make them identical.

    In summary:
    If the sequence is c-terminal, block the n-terminus by acetylation.
    If the sequence is n-terminal, block the c-terminus by making it an amide.
    If the sequence is internal, block both ends with acetylation and amidation.


    Q: If my peptide is 95% pure, what is in the other 5%?
    A: Peptide purity is determined by reverse phase HPLC using a standard gradient (1% per minute) established by NEP. During synthesis, the coupling reaction of one amino acid to another is not always 100% efficient, causing a variety of deletion sequences to be generated. Most of the deletion sequences are purified out, but a few may have similar chromatographic characteristics as the target peptide. These remain in the peptide sample and account for the few percent of impurities.


    Q: How do I reconstitute my peptide?
    A: Solubility of peptides depends upon peptide sequence. First try sterile water and sonication (1-10mgs/ml). If this fails, count the number of basic residues (Lys, Arg, His and free N-terminus), then count the number of acidic residues (Glu, Asp and free C-terminus). If there are more basic residues add .1N acetic acid dropwise until solubilized. If there are more acidic residues add .1N ammonium hydroxide dropwise until solubilized. When using buffers in your experiments it is recommended that you do not add salts until the peptide is in solution, since salts may hinder solubility. If you can not solubilize your peptide, you may also try organic solvents such as DMSO, acetonitrile, or DMF.


    Q: What is PepTrend©?
    A: PepTrend© is a software program designed by NEP to facilitate peptide design and synthesis during peptide production. Every sequence is tested in the three areas of peptide production, including synthesis, cleavage and purification. PepTrend© uses decades of scientifically proven data, along with general trends found during the synthesis of thousands of peptides to warn of potential problems during any of the three areas of synthesis. The software allows NEP to provide the highest level of service for our customers. NEP can instantly warn customers of potential problems while seeking alternate synthesis strategies before production starts.


    Q: What is net peptide content and what does it mean?
    A: The weight of dry peptide doesn’t consist of peptide only, but includes non-peptide components such as water, absorbed solvents, counter ions and salts. Net peptide content is the actual percent weight of peptide. This number may vary, from 50-90 percent, depending on the purity, sequence and method of synthesis and purification. Do not confuse peptide content with purity, they are two distinctly separate things. Purity is usually determined by HPLC. Purity defines the percent of sample that is the correct peptide sequence, while net peptide content only gives information on the percent of peptide versus non-peptide components. Net peptide content is accurately found by performing amino acid analysis or UV spectrophotometry. This information is important when calculating concentrations of peptide during sensitive experiments. If required, please ask NEP.


    Q: What purity do I need for my experiments?
    A: The final purity of a peptide is very important and depends on the type of experimentation you are doing:
    • For non-sensitive screening assays crude or >75% is recommended.
    • For immunogen grade >85% is recommended.
    • For receptor/ligand studies, bio-assay studies, or cell studies >95% is recommended.
    • For structural studies >98% is recommended.


    Q: How do I store my peptides?
    A: Peptide Storage: If possible, peptides should always be stored in lyophilized form at -20° C. This will prevent bacterial degradation, secondary structure formation, oxidation and other modifications for up to several years. Peptides in solution are less stable, so it is strongly recommended that you use sterile water or sterile filtration during reconstitution. If there are any methionine, cysteine, or tryptophan residues, use oxygen free solvents to prevent oxidation. When using frozen solutions, it is recommended to divide into aliquots to prevent damage caused by several freeze-thawing cycles. Recommended pH range should be between 3-6.

    Long Term Storage: (3 months-5 years) Lyophilized powder, frozen and desiccated at -20° C.
    Medium Term Storage: (0-3 months) Frozen liquid (-20° C) or refrigerated lyophilized powder.
    Short Term Storage: (<1 week) Refrigerated liquid or refrigerated lyophilized powder.


    Q: What is the maximum peptide length you can produce?
    A: New England Peptide, Inc. routinely synthesizes peptides >70 amino acids in length. The longest peptide (protein) we have made synthetically has been 120 amino acids long.


    Melanotan-1 ([Nle4, D-Phe7]α-MSH - under its generic name "afamelanotide") was approved on May 5, 2010 by the Italian Medicines Agency (AIFA - Agenzia Italiana del Farmaco) as a medication for those afflicted with the orphan disease erythropoietic protoporphyria (EPP). The drug is used to induce photoprotective dermal eumelanin pigmentation and thereby reduce the effects of painful, burning dermal photosensitivity caused by the disease. 1 2 This marks the first time ever that a governmental health regulatory agency has given approval for this drug for usage in human medicinal therapy. For EPP treatment the drug will be marketed under the trade name "Scenesse"® a registered brand name trademark of Clinuvel Pharmaceuticals Ltd. (formerly EpiTan Ltd. 3 4).

    What are the drugs Melanotan-1 and Melanotan II?

    Based upon a quote of the Arizona Cancer Center found at sunless.com (on June 14th, 1999):

    Melanotan-1 and Melanotan II are both synthetic versions of α- melanocyte stimulating hormone that were created, synthesized and developed at The University of Arizona and the Arizona Cancer Center. Melanotan-1 is a linear, full length peptide (containing all 13 amino acids). Melanotan II is a shortened, circular version of melanotan-1. Both Melanotan-1 and Melanotan II develop dermal pigmentation without sun exposure but because Melanotan II had libido enhancement and spontaneous erections as side effects, it is now being developed as a sexual and erectile dysfunction drug.

    To be particularly clear regarding Melanotan-1 (due to previous incorrect news reports) it should be noted that it does not cause sexual arousal nor have erectile effects. These aspects are only found in the drugs Melanotan II and bremelanotide.

    SIDE EFFECTS

    http://articles.muscletalk.co.uk/article-melanotan.aspx


    For all answers and questions please post in this thread. Will be a lot easier than having 10 damn MT threads every other day lol.
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    You expect me to read all of that?
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    Some very important information with regards to melanotan-1 and melanotan II peptides, please read carefully:

    In light of recent reports of strong allergic reactions and heightened concerns over Mannitol adulteration, I have done some extensive investigation, online, with experts in the field of peptides and with labs I have contacted in China and would like to share that information here:

    >>>>>>>>>>>>>>>>>>>>>>>>>>>>>
    Mild to Strong allergic reactions to injections!!!
    >>>>>>>>>>>>>>>>>>>>>>>>>>>>>

    Recently there has been a series of reports from people on this board, new and old users alike, with regards to extreme cold/allergy like symptoms including but not limited to, cough, sore throat, runny nose, dry/itchy eyes, swelling at point of injection, excessive mucus down throat (post nasal drip which also is probably causing the cough) and headaches.

    All of these things and more are indicative of allergic reactions to the injection which is usually caused by impurities in the peptide or diluent. The more impurities the greater the reaction is that you will have. The peptide itself, if it was ever possible to have 100% pure peptide which it is not, would not produce such an effect. Therefore, no matter 98% or even 99% pure, you will still have a chance of having some allergic like reaction as is the case with most injections of any kind. This kind of reaction is very pronounced in influenza injections where you are being injected with a dead virus. In some cases you may even have a slight fever but should go away quickly.

    There is, in almost every vial of any peptide or drug, the possibility, and therefore the need to assume that, there is some small amount of bacteria. You can verify this yourself just by checking any assay report you have ever seen on any peptide. The amount is very small but it is there. In most cases, if there are bacteria, it is more then likely dead from all the binding agents, reagents and other processes that the peptide goes through but even dead bacteria, like a virus, will still produce a larger reaction then just some other inert contamination.

    The peptide also breaks down over time causing more of its content to become foreign. That is to say, when the peptide is no longer in its proper chain to mimic alpha-Melanocyte Stimulating Hormone (a-MSH) then it is seen as a foreign object to your body and will also cause an allergic reaction.

    Could Mannitol in my peptide be causing this reaction? Well, yes and no. No, because pure Mannitol will also not have this kind of effect but the Mannitol itself can also have some contamination. Two substances in the vial creates double the chance for contamination as the substances are added together after the peptide is complete.

    If you are having some light allergic like reactions to the peptide, this is rather normal and many times will actually go away or not be noticeable after taking the peptide for some time. Some people take an antihistamine prior to use so as to avoid this issue. However, it may happen again if you switch suppliers or get a different batch that could potentially have different material in it that your body is not used to. Your allergic reaction may actually be intensified if you take the peptide on a very irregular basis. Say you take some one day, forget for a couple of days and then take it again or maybe you do that on purpose, only one shot a week or something. These allergic reactions will be far less if you are taking the peptide regularly and your body builds up a tolerance to those foreign bodies.

    If you are having severe allergic reactions, “Heavy coughing, lots of mucus in your throat, constant headaches, rash, itching or other strong effects to the peptide, you should:

    1)Stop taking the peptide.
    2)Inject yourself with just straight diluent to rule that out as the cause.
    3)If no reaction to the diluent, throw away or try to return your vials to your supplier.
    4)Alternately you can have your peptide tested for purity which is a good idea to do regardless. There have been a few people already on this board who have had peptide tested and you can ask them where they had that done. Alternately, if enough people wish to do this, I can make arrangements with a testing facility where you can send the peptide for testing. I can try to work out a deal whereby you can say you are with Melanotan.org and get a discount. I cannot say for sure but I am guessing that the cost will be much lower then any in like the US or EU, I am guessing around $50 but you will have to ship your vial to China. Any way, if anyone wants to do that let me know and if enough people do I will see what I can work out.

    The longer you or your supplier stores the peptide before end use, the greater the chance for break down contamination and therefore allergic reaction. Depending upon the conditions, storage, usage procedures and age of your peptide, all can cause mild to severe reactions. Therefore, keep your environment as sterile as possible, do not use old needles, always swab the top of your vial before injection, do not keep your peptide for longer then a year in freezer and do not keep your mixed peptide in the refrigerator for more then a month. If you have accidentally left your mixed peptide out for a day or two, even over night, you are best off to throw it away.

    >>>>>>>>>>>>>>>>>>>>>
    More about Mannitol
    >>>>>>>>>>>>>>>>>>>>>

    How do I know if I have Mannitol in my peptide? You can view the peptide in your vial to help make this determination as 10mg of peptide is a very small amount of material. If the content of your vial is dense and filling a large portion of the vial then it probably has a filler. When the peptide is lyophilized it will leave behind a very porous column of the peptide in the vial which can be as high as the water that was originally added but also could have broken down to a dusting of material at the bottom of the vial. If you have any further doubt, it will require a very precise scale, you can take an empty vial with stopper and cap then weigh it. Next take a full vial and weigh it and calculate the difference. It should only be 10mg.

    But some vials have more then others; some are nearly 50% full while others are only slightly higher then the bottom of the vial. Some are clumpy, some are compact and others are balled up. Would some have Mannitol and others not? Once again, if there is more then a very light dusting at the bottom, with the bottom of the vial actually being able to be seen, or an extremely porous column of white, or something somewhere between, then you probably have Mannitol. In fact, if your vial has been shipped or rolled around, you may not see anything at the bottom at all, it may all be stuck to the sides of the vial, looking like very little powder around the sides. Some labs will add more Mannitol then others, and some vials are more dry then others which also creates a variance in the look. Attached are some pictures of the various ways that your peptide may look. Please note that the first three are all (as confirmed by the labs who provided them) with Mannitol and the last one is the only one without any Mannitol.

    Will Mannitol hurt me? Mannitol has been approved even by the FDA as an additive to drugs. In large quantities it can have some bad effects but these are much larger amounts then you will find in the peptides. However, again, the Mannitol itself can have contaminants and therefore can double your chance, risk, of having allergic like reactions. In addition, there is no reason to add Mannitol to melanotan-1 or Melanotan II other than to increase the volume.
    Mannitol itself is not going to hurt you but the effects of it combined with the peptide is not known. It may also contain other contaminants that can affect on your body and it is used with drugs to help them cross the Blood Brain Barrier. Melanotan II already can do this but melanotan-1 cannot so if your melanotan-1 has Mannitol then you are negating the Barrier and possibly the reason you are taking melanotan-1 in the first place. Better to press your supplier for non Mannitol peptide and do not trust that it does not have Mannitol unless it is as I described above and like the last picture shown.

    Why is anyone adding Mannitol to the peptide? To make the content of the vials look greater the actual as people had complained that their vials did not contain anything or very little.

    >>>>>>>>>>>>>>>>>>>>>>
    NEW INFORMATION REGARDING MANNITOL SIDE EFFECTS
    >>>>>>>>>>>>>>>>>>>>>>

    Subsequent to my initial posting, I have found more information, related to Mannitol, that I felt should be posted. Granted that there are many regulated medicines on the market that contain Mannitol but those are regulated, the amount of Mannitol is known and the affect of the Mannitol with these drugs are known. It is also interesting to note these side effects of Mannitol alone when compared to many of the unusual sides effects being reported on this site.

    Side Effects of Mannitol - for the consumer


    Mannitol

    All medicines may cause side effects, but many people have no, or minor, side effects. Check with your doctor if any of these most COMMON side effects persist or become bothersome when using Mannitol:
    Increased urination; nausea; runny nose; vomiting.
    Seek medical attention right away if any of these SEVERE side effects occur when using Mannitol:
    Severe allergic reactions (rash; hives; itching; difficulty breathing; tightness in the chest; swelling of the mouth, face, lips, or tongue); blurred vision; chest pain; chills or fever; confusion; decreased alertness; difficulty urinating; extreme dizziness; extreme thirst or dry mouth; fast or irregular heartbeat; headache; muscle cramps; pain, redness, or swelling at the injection site; weakness.
    Top

    For the professional


    Mannitol Injection

    Reactions are infrequent and may include:
    Metabolic: fluid and electrolyte imbalance, acidosis, dehydration.
    Gastrointestinal: dryness of mouth, nausea, vomiting, diarrhea.
    Genitourinary: osmotic nephrosis, urinary retention.
    Central Nervous System: headache, convulsions, dizziness.
    Special Senses: Blurred vision, rhinitis.
    Cardiovascular: pulmonary edema, hypotension, hypertension, tachycardia, angina-like chest pains.
    Dermatologic: skin necrosis, thrombophlebitis.
    Hypersensitivity: urticaria.
    Miscellaneous: thirst, arm pain, chills, fever.

    Information obtained from www.drugs.com


    Mannitol Added

    Mannitol Added

    Mannitol Added
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    wall of text/10

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    Originally Posted by benzz View Post
    is melanotan legal?
    Its not FDA approved, but in most places its not Illegal.

    Originally Posted by NillaWafer View Post
    You expect me to read all of that?
    Yes you can also post questions and I'll answer as we go. I just want this to be the official thread. Feel free to add any more info.
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    cliffs?

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    Originally Posted by swearujacked View Post
    wall of text/10
    Originally Posted by bvalks View Post
    cliffs?
    I know guys, but give it a chance. If you are serious about this stuff, YOU need to read it. Feel free to ask questions. Thats what i want with this thread.
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    Originally Posted by eXistenceLies View Post


    Yes you can also post questions and I'll answer as we go. I just want this to be the official thread. Feel free to add any more info.
    i read the whole thing, im actually interested in this

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    Originally Posted by NillaWafer View Post
    You expect me to read all of that?
    Don't read it, inject yourself with crap and get an infection. Not a single **** will be given.

    Top thread.

  14. #14
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    Originally Posted by eXistenceLies View Post
    I know guys, but give it a chance. If you are serious about this stuff, YOU need to read it. Feel free to ask questions. Thats what i want with this thread.
    whats it for...

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    Originally Posted by benzz View Post
    i read the whole thing, im actually interested in this
    You have super powers brah? lol

    Originally Posted by xplicid01 View Post
    whats it for...
    Injections to make you tanner.
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    Hey thanks for posting all this. I was wondering if there is something that does the opposite? There would be a huge market, if so.

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    Originally Posted by strugglingfarts View Post
    Hey thanks for posting all this. I was wondering if there is something that does the opposite? There would be a huge market, if so.
    To make you white? Yea go stay in your parents basement for one year and not come out. I think that will reverse the effects. Cmon bro....Thats the dumbest question. Trying to make this a serious thread.
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  18. #18
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    Remember tons of more info at the site posted at the very first post. I just copied the most important information regarding this stuff.
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    what if I can't put it in the fridge, what other options do I have

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    Originally Posted by eXistenceLies View Post
    To make you white? Yea go stay in your parents basement for one year and not come out. I think that will reverse the effects. Cmon bro....Thats the dumbest question. Trying to make this a serious thread.
    How is that a dumb question? You don't think it would be possible to lighten someones skin? They already do it via topicals.

  21. #21
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    why the fhuck would u go through all the trouble to inject urself with some **** when u can just go outside.........

    Nd im half black i get dark in 20 mins

    U mad OP???
    I rep back

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    brb spontaneous erections

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    Originally Posted by eXistenceLies View Post
    You have super powers brah? lol



    Injections to make you tanner.
    i read fast u mad?

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    Originally Posted by Mixed_Breed View Post
    why the fhuck would u go through all the trouble to inject urself with some **** when u can just go outside.........

    Nd im half black i get dark in 20 mins

    U mad OP???
    sry brah, its not so easy for some people

    I'm irish and SICKLY pale in the winter, can't exactly lay out for two weeks in 20 degree weather, which is how long it would take for me to get a decent tan

    besides insulin needle's like a metal hair, don't be a bitch

  25. #25
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    Originally Posted by Anubis18 View Post
    what if I can't put it in the fridge, what other options do I have
    And why not?


    Originally Posted by Mixed_Breed View Post
    why the fhuck would u go through all the trouble to inject urself with some **** when u can just go outside.........

    Nd im half black i get dark in 20 mins

    U mad OP???
    Well since you are half black, you must not know that some people can't tan and instead burn or turn red as a lobster. This stuff is there to make you tan without the use of the sun/and or bed. They say to use the bed or sun to maximize the tan. They even mention you only need to spend half the time in the sun/bed than when using this stuff. Just letting you know that some skin tones can't tan. It would take me all summer to get a slight color to my skin. IMO thats a waste and not worth my time. I hate spray tan as well. Too much of a hassle.
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    Originally Posted by benzz View Post
    i read fast u mad?
    Not mad brah, just surprised. GJDM! Feel free to ask away. I guess i should post up my before and after pictures... as well




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  27. #27
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    sigh this **** is illegal in australia im pretty sure.
    If you have any questions at all go here - http://www.formspring.me/Asinima

  28. #28
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    Originally Posted by eXistenceLies View Post
    And why not?
    .
    not the easiest thing to explain to people who find strange glass vials in the freezer

    I don't want to be super dark so I'm thinking I might get some and just use it for a few days and toss the rest, would it be alright to throw it in a cooler with ice for 3-4 days?

  29. #29
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    What a dumb thing to do
    "Losers always whine about their best. Winners go home and **** the prom queen."
    --Sean Connery (The Rock)

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    holy god what a waste of time/money

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