Take a look at this article:
They tested, among other things, testosterone levels of males fed various levels of dietary zinc. To sum it up: The findings suggest that serum testosterone concentrations, seminal volume, and total seminal zinc loss per ejaculate are sensitive to short-term zinc depletion in young men.
Zinc is lost rapidly after sex. Would it be reasonable to think that frequent sex and/or fappage might lead to reduced zinc levels, and hence, reduced testosterone levels? Short term studies seem to say no, but what about long term constant zinc depletion?
Thread: Zinc and Testosterone?