Ejaculation Frequency vs. Testosterone Level:
1. http://www.ncbi.nlm.nih.gov/pubmed/1...?dopt=Abstract - "The purpose of this study is to gain understanding of the relationship between ejaculation and serum testosterone level in men. The serum testosterone concentrations of 28 volunteers were investigated daily during abstinence periods after ejaculation for two phases. The authors found that the fluctuations of testosterone levels from the 2nd to 5th day of abstinence were minimal. On the 7th day of abstinence, however, a clear peak of serum testosterone appeared, reaching 145.7% of the baseline ( P < 0.01). No regular fluctuation was observed following continuous abstinence after the peak. Ejaculation is the precondition and beginning of the special periodic serum testosterone level variations, which would not occur without ejaculation. The results showed that ejaculation-caused variations were characterized by a peak on the 7th day of abstinence; and that the effective time of an ejaculation is 7 days minimum. These data are the first to document the phenomenon of the periodic change in serum testosterone level; the correlation between ejaculation and periodic change in the serum testosterone level, and the pattern and characteristics of the periodic change." also in http://www.ncbi.nlm.nih.gov/pubmed/1...ystem2.PEntrez
2. http://www.ncbi.nlm.nih.gov/pubmed/1...?dopt=Abstract - "This current study examined the effect of a 3-week period of sexual abstinence on the neuroendocrine response to masturbation-induced orgasm. Hormonal and cardiovascular parameters were examined in ten healthy adult men during sexual arousal and masturbation-induced orgasm. Blood was drawn continuously and cardiovascular parameters were constantly monitored. This procedure was conducted for each participant twice, both before and after a 3-week period of sexual abstinence. Plasma was subsequently analysed for concentrations of adrenaline, noradrenaline, cortisol, prolactin, luteinizing hormone and testosterone concentrations. Orgasm increased blood pressure, heart rate, plasma catecholamines and prolactin. These effects were observed both before and after sexual abstinence. In contrast, although plasma testosterone was unaltered by orgasm, higher testosterone concentrations were observed following the period of abstinence. These data demonstrate that acute abstinence does not change the neuroendocrine response to orgasm but does produce elevated levels of testosterone in males."
3. American population testosterone level dropped about 50 ng/dl for men at around age 64-65 between 2 groups of men born in 1920-1924 and 1930-1934, according to in http://jcem.endojournals.org/cgi/reprint/92/1/196. When the 1920-1924 group reached the median age 65, their mean testosterone level was 500 ng/dl; when the 1930-1934 group reached the median age 56 and 64, their mean testosterone was 529 ng/dl and 444 ng/dl, respectively. The 1930-1934 group has a testosterone drop rate at about 10.65 ng/dl per year during ages 56-64. This report also shows that the testosterone drop rate generally becomes faster for the men from 55 to 65. If we use the same annual drop rate of the 1930-1934 group, the extrapolated, averaged testosterone level of of 20 year old men born during 1930-1934 should be about 911 ng/dl. Assuming that the mean 20-year old testosterone level for both groups are the same is about 911 ng/dl, the overall-averaged testosterone drop for the 1920-1924 group is about 9.13 ng/dl/year, while the overall-averaged testosterone drop for the 1930-1934 group is about 10.61 ng/dl/year I suspect the higher masturbation/ejaculation frequency in the younger generation after the 60's sexual revolution resulted in a higher cortisol/ prolactin level (or faster ageing of the hypothalamus-pituitary-adrenal and -testicular axis) accelerates the testosterone drop, since some high-frequency over-masturbation young men experience male menopause (andropause) between ages 20-30.
4. Our observations for healthy ejaculation frequency (or refraction times) vs age, in order to maximize the testosterone level, are:
1. 4-7 times per week for teenagers of age 16-19.
2. 3-6 times per week for young men of age 20-25.
3. 3-5 times per week for young men of age 25-30.
4. 2-4 times per week for men of age 30-45,
5. 1-3 times per week for men of age 45-60,
6. 1-2 times every 10 days for men of age 60-70.
7. No more than once a week days after age 70
Lovemaking orgasms stimulate the pituitary to release oxytocin for a faster recovery; while masturbation may not help the pituitary to release enough oxytocin. Therefore, the masturbation ejaculation frequency should be limited to the low limit as possible as you can. If your ejaculation orgasm produces sexual exhaustion symptoms, your ejaculation frequency should be lower than the listed above. The actual ejaculation frequency is associated the refraction (recovery time) of the hypothalamus-pituitary-adrenal, -thyroid and -testicular axis. The energetic time (full recovery with powering up) for a man having a morning (8 AM) testosterone level at 350-400 nd/gl is about 7 days when his testosterone level reach its peak, over 500 ng/dl. If he keeps this ejaculation frequency, he will likely maintain his testosterone level around 450-550 ng/dl, thereafter. A man with a high testosterone level of 700 ng/dl or higher can re-erect the penis in a few minutes after ejaculating if his pituitary doesn't overshoot his prolactin level, but it doesn't mean he can ejaculate again. This is because the ability of penile re-erection can be associated with the dopamine nervous action on the pituitary oxytocin and prolactin release and the stimulation of testosterone, nitric oxide, prostaglandin E1 and prostaglandin E2 stimulation on the prostate and penile erectile nerves. Prostaglandin E2 can re-erect the post-ejaculating penis again when the oxytocin level is high and the prolactin level is not overshooting out of the range. The prostaglandin E2 induced erection usually accompanies a little pains in the prostate, urethra and penis. Even if you can re-erect your penis for sex again, the post-ejaculation elevating cortisol and prolactin will start to lock up the adrenal and testicular function to drop your DHEA and testosterone production few hours after the first ejaculation.
Like psychological stress or exercise-induced stress, sex-induced stress induces excessive cortisol, prolactin and norepinephrine/epinpehrine release to lock up the hypothalamus-pituitary-adrenal and -testicular axis during sex or few hours later after orgasm or ejaculation. The neuro-immune-endocrine responses to sex-induced stress can be delayed until cortisol starts to exert its effects on the adrenal cortex, prolactin on the pituitary-testicular axis, and norepinephrine/epinephrine and its induced prolstaglandin E2 overheat the hypothalamus, pituitary, adrenal glands, testicles (ovaries), prostate, liver, lungs, heart and pancreases (for a high glucogan release) via the alpha- and beta -andrenergic receptors. For the same reason, people with chronic stress, no matter from which sources, generally have a lower testosterone level. Over-trained athletes and soldiers and psychologically stressed-out persons are not exceptional. Testosterone and norepinephrine can positively or negatively interact each other through their receptors. Initially, testosterone induces norepinephrine release to give your arousal and heat, and then excessive norepinephrine with a high level of proactin and cortisol reduces testosterone release; on the other hand, without the inhibition effects of excessive prolactin and cortisol, norepinpehrine stimulates testosterone release via action on both alpha- and beta-andrenergic receptors in the adrenal and testicles (ovaries) when the testosterone level is too low. To avoid the inhibition effects of excessive prolactin and cortisol, you need a normal dopamine-hypothlamaus-pituitary-adrenal function. Any way, there are a lot of researches backing up the claim about the interaction among the nervous, immune and endocrine system:
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The following research suggests the inflammatory cytokine IL-1beta and its induced prostaglandin E2 can stimulate the hypothalamus to induce release of norepinephrine, dopamine, and serotonin as a feedback control.
This article suggest norepinephrine control the feedback of LH secretion in the hypothalamus-pituitary-testicular axis - http://www.popline.org/docs/0188/761130.html
You will get more explanation in the following about the role of norepinephrine and epinephrine in the sexual arousal, orgasm responses, sexual exhaustion symptoms, and neuro-immune function.