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  1. #1
    Registered User Swifto's Avatar
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    Lj100 WILL NOT raise Testosterone

    Products containing Lj100 WILL NOT raise Testosterone, its that simple.

    The "studies" posted by product companies are not "studies". Their articles written by manufacturers and patent holders made to look like "studies".

    I have never seen a single subject that has showed a raise in total testosterone using any Lj100 based product. The bloodwork I have seen, when AndroGenerator was used over at Outlawmuscle.com, showed no increase whatsoever.

    There isnt a single published study confirming Tribulas or Lj100 raise total testosterone published anywhere reputable, such as Pubmed/Medline.

    Any product containing such ingredients, promising to raise total testosterone, are simply a waste of money.

    Websites such as mindandmuscle.net and anabolicminds.com, with some VERY intelligent members and supplement designs share this very view.

    The one "study" that has been published is full of errors and is laughable. If anyone posts it, I'll state why.

    I have used Lj100/Trib based products (Tribex, AndroGenerator) and havent witnessed a raise in testosterone. I know this as a get severe acne if my body even whiffs a a raise in total T. I also have a small case of gyno that has never been aggrivated using such products. Even at massive doses I have never experienced any changes.

    My advice: If you want to raise total T, use a low dose of an AI or SERM. AI's work particularly well.
    Last edited by Swifto; 07-03-2008 at 06:34 AM.
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  2. #2
    Just call me Lucifer THEHUGE's Avatar
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    Angry wtf?

    Sorry,I surely read it wrong.YOU GOT NO TESTOSTERONE RISE BECAUSE YOUR ACNE HASN'T GOTTEN WORSE NOR YOU'VE HAD GYNO?????
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  3. #3
    Carb Depleted as **** G0re0bsessed's Avatar
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    Originally Posted by THEHUGE View Post
    Sorry,I surely read it wrong.YOU GOT NO TESTOSTERONE RISE BECAUSE YOUR ACNE HASN'T GOTTEN WORSE NOR YOU'VE HAD GYNO?????
    Thats what I got from his post too... LMAO
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  4. #4
    Registered User Swifto's Avatar
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    Originally Posted by G0re0bsessed View Post
    Thats what I got from his post too... LMAO
    Bloodwork has also been posted numerous times regarding lj100 and shown no raise whatsoever.

    Me having the odd breakout or my gyno being irritated would be a clear indication, to me, that my total testosterone would have raised even the slightest bit.

    Lj100 claims to raise your total testosterone over "400%". With the sort of raise, I difference would be noticed using Lj100.
    Last edited by Swifto; 07-03-2008 at 05:05 AM.
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  5. #5
    Just call me Lucifer THEHUGE's Avatar
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    Originally Posted by Swifto View Post
    Bloodwork has also been posted numerous times regarding lj100 and shown no raise whatsoever.

    Me having the odd breakout or my gyno being irritated would be a clear indication, to me, that my total testosterone would have raised even the slightest bit.

    Lj100 claims to raise your total testosterone over "400%". With the sort of raise, I difference would be noticed using Lj100.
    Please provide a link for those numerous bloodworks. The 400% rise was NOT in vivo but on hemogenated leydig cells.Nobody claimed a similar increase in vivo.
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    I stopped reading at arnt. Not saying I totally disagree with the thread though.
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  7. #7
    Registered User jeff3kids's Avatar
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    Gents, this is not complicated. Google the referenced studies. Swifto is correct. The ONLY place you will find the "studies" on all of the internet will be from highly biased company's.

    You don't have to believe us, do the search yourselves. LJ100 is a joke at best,and a potential health hazard. We wont know for sure until a real study takes place.

    In the mean time, I know Androgenerator did nothing for me except give me a nasty case of the runs, and it contains the purest lj100 according to SSE.

    There is another good guy on another site who actually posted blood work after 4 weeks of massave doses of the same product. His total test came back at 150.

    That situation turned so bad that ross actually posted the mans address on an OPEN SOURCE steroid forum. He did this TWICE.

    I'm not out to "get" anyone. But I know what I know without question. There are really good test boosters available. lj100 is not one of them.
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  8. #8
    Registered User jeff3kids's Avatar
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    BTW, this is what a real study looks like from a well respected journal. Thanks to Tawney76 for the original link.

    http://jcem.endojournals.org/cgi/rep...469.pdf?ck=nck
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    Question ?

    Originally Posted by jeff3kids View Post
    BTW, this is what a real study looks like from a well respected journal. Thanks to Tawney76 for the original link.

    http://jcem.endojournals.org/cgi/rep...469.pdf?ck=nck
    could you please explain me what this has to do with lj100?
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  10. #10
    hustlehands Hansolo261's Avatar
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    Ok from my understanding normal level of testosterone in your bloodstream is between 350 and 1,000 nanograms per deciliter (ng/dl). Now when some people maybe around 400 other could be higher now lets just say you are around 350 and you take a test booster it may get you to around 600 or greater and you may expeirence some benefits. Now if you are around 800 already the test booster may not do much for you.
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    *PUBMED* Study demonstrating the ANDROGENIC effects of Long Jack:

    Effects of Eurycoma longifolia jack on laevator ani muscle in both uncastrated and testosterone-stimulated castrated intact male rats.

    Ang HH, Cheang HS.
    School of Pharmaceutical Sciences, University Science Malaysia, Minden, 11800, Penang, Malaysia. hhang@usm.my

    It has been reported that Eurycoma longifolia Jack commonly known as Tongkat Ali has gained notoreity as a symbol of man's ego and strength by the Malaysian men because it increases male virility and sexual prowess during sexual activities. As such, the effects of 200, 400 and 800 mg/kg of butanol, methanol, water and chloroform fractions of E. longifolia Jack were studied on the laevator ani muscle in both uncastrated and testosterone-stimulated castrated intact male rats after dosing them for 12 consecutive weeks. Results showed that 800 mg/kg of butanol, methanol, water and chloroform fractions of E. longifolia Jack significantly increased (p<0.05) the leavator ani muscle to 58.56+/-1.22, 58.23+/-0.31, 60.21 +/-0.86 and 62.35 +/-0.98 mg/100 g body weight, respectively, when compared with the control (untreated) in the uncastrated intact male rats and 49.23+/-0.82, 52.23+/-0.36, 50.21+/-0.66 and 52.35+/-0.58 mg/100 g body weight, respectively, when compared to control (untreated) in the testosterone-stimulated castrated intact male rats. Hence, the pro-androgenic effect as shown by this study further supported the traditional use of this plant as an aphrodisiac.

    PMID: 11693547 [PubMed - indexed for MEDLINE]

    *PUBMED*Study demonstrating the Aphrodisiac effects of Long Jack:

    Eurycoma longifolia Jack enhances libido in sexually experienced male rats.Ang HH, Sim MK.
    School of Pharmaceutical Sciences, University of Science Malaysia, Penang, Malaysia.

    The effects of Eurycoma longifolia Jack were studied on the libido of sexually experienced male rats after dosing them with 200, 400 and 800 mg/kg body weight twice daily of different fractions of E. longifolia Jack for 10 days. Results showed that E. longifolia Jack produced a dose-dependent increase in mounting frequency of the treated animals with 400 mg/kg of chloroform, methanol, water and butanol fractions resulting in mounting frequencies of 5.3 +/- 1.2, 4.9 +/- 0.7, 4.8 +/- 0.7 and 5.2 +/- 0.1, and 800 mg/kg further increased them to 5.4 +/- 0.8, 5.4 +/- 0.8, 5.2 +/- 0.6 and 5.3 +/- 0.2 respectively but there were no erections, intromissions, ejaculations or seminal emissions during the 20-min observation period which allowed for the measurement of sexual arousal reflected by mounting frequency uninfluenced by other behavioural components. This study provides evidence that E. longifolia Jack is a potent stimulator of sexual arousal in sexually vigorous male rats in the absence of feedback from genital sensation.

    PMID: 9353636 [PubMed - indexed for MEDLINE]

    Another:

    Eurycoma longifolia Jack enhances sexual motivation in middle-aged male mice.Ang HH, Lee KL, Kiyoshi M.
    School of Pharmaceutical Sciences, University Science Malaysia, Minden, Penang, Malaysia. hhang@usm.my

    Eurycoma longifolia Jack was investigated for sexual motivation activity in adult, middle-aged male mice and in retired breeders, using the modified open field and the modified runway choice methods. Each mouse received 500 mg/kg of one of 4 fractions of E. longifolia Jack, viz. chloroform, methanol, butanol, and water, whereas the mice in the control and yohimbine groups received 3 ml/kg of normal saline and 30 mg/kg of yohimbine daily respectively for 10 d. The results show a transient increase in the percentage of male mice responding to the right choice after chronic consumption of the fractions with 50 percent of the adult middle-aged male mice treated with E. longifolia Jack and yohimbine scoring the right choice after 8 and 5 days post-treatment respectively. In conclusion, this study has shown that E. longifolia Jack continues to enhance sexual motivation in adult, middle-aged male mice and in retired breeders.

    THE ANABOLIC EFFECTS OF LJ100

    For a printable copy of this study, please click here: http://www.source-1-global.com/pdfs/...al-Studies.pdf

    Sareena Hanim Hamzah & Ashril Yusof
    Department of Exercise Physiology, Sports Centre, University of Malaya, Kuala Lumpur

    Introduction
    Eurycoma longifolia (LJ100?) is a tall shrub tree of a Simaroubaceace family and is commonly found along the hilly jungle slopes of Malaysia. It has been used for years as a traditional medicine to treat fever, ulcer, malarial, swelling, reduce high blood pressure and fatigue. However, LJ100 is better known for its aphrodisiac properties. In a clinical study by Ismail (2002), he demonstrated that this herb enhanced sexual activities and increased free testosterone levels in men. Increases in testosterone levels is associated with an improvement in fat free mass, muscle size and muscle strength in men (Brodsky, 1996; Bhasin, 1997), which could be further amplified by strength training (Bhasin, 1996). In this study, the effect of LJ100 water-soluble extract on body composition and muscle size in men will be measured.

    Methods
    Fourteen healthy adult males (age 25.64 ? 3.73 years) received either 100 mg/day LJ100 water-soluble extract (n = 7) or placebo (n = 7) for 8 weeks. Simultaneously, both groups performed an intensive strength-training program with initial load of 60% repetition maximum (RM), which was carried out on alternate days. A total of 10 exercises, which make up the circuit, were catered towards providing a total lower body and upper body workout. Each workout was done in two sets of 10 repetitions with 1-minute rest in between. The loads were gradually increased 10% per week. Body composition measurement using skin fold test was taken at two sites as recommended by McArdle (1993). A standard strength test that comprised of 1 RM test was administered on the subject to determine their strength. The upper limb strength was measured by determining their ability to resist maximum load using the shoulder press machine (Nautilus, USA) following the American College of Sports Medicine (ACSM, 2001) standard measurement procedure. The arm circumference measurement was taken using a measuring tape at proximal 1/3rd of the arm. Electromyography reading of the isometric contraction of bicep muscle was taken using the surface electrodes. Subjects were instructed to perform an isokinetic flexion of the elbow using free barbells with load of 10 kg for the durations of 5 seconds. The mean amplitude was analyzed using the MyoResearch Software (Noraxon, USA).
    All the measurements were taken 1 day prior to supplementation (LJ100 and placebo) and training period, and 1 day after the completion of 8 weeks experiment. All data were analyzed using the Statistical Package for Social Science (SPSS) computer software version 10.0 (2000) for t-test, means and standard deviation. Statistical significance was established at p<0.05.

    Results
    The results for fat free mass, fat mass, 1 RM, arm circumference, and sEMG of both groups are shown in Table 1.

    Table 1. Average fat free mass, fat mass, 1 RM test, arm circumference and sEMG of the group consuming LJ100 water soluble extract and placebo before and after the period of supplementation and training program


    * Results of mean ? SD for pre and post experiment showed significant difference (p<0.05)

    The fat free mass of the group supplemented with LJ100 water-soluble extract showed a significant increment of approximately 2.1 kg. There were no significant changes in fat free mass in placebo. Body fat percentages were significantly decreased in treatment and placebo. However, a greater decrement was shown in treatment compared to placebo i.e. 9.14% and 6.57%, respectively. The 1 RM test muscle strength test showed an increase in gross muscle power in both groups. The treatment group showed a greater increment in strength compared to placebo i.e. 6.78% and 2.77%, respectively. The mean arm circumference in treatment group increased significantly by 1.8 cm following the supplementation while no significant changes observed in placebo group. The mean sEMG reading of the treatment and placebo showed a significant decrement in values after going through the exercise program. However, the treatment group showed 2.92% higher reduction in electrical activity of the muscle measured at the end of the experiment period compared to placebo (25.70% and 22.78%, respectively). During and after the administration of LJ100, no adverse effects were noted within the treatment group.
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    Discussion/ Conclusion
    In this study, although the testosterone level was not measured during the test period, an increased in fat free mass in treatment group may be linked to the rise in steroidal hormones in the body. The percentage of body fat appeared to decrease in both groups, this finding is in agreement with a study by Brodsky (1996). However, the further decrease in fat mass by the treatment group could be explained by the higher metabolic rate after consuming LJ100. The increment in muscle strength with strength training in both the treatment and placebo groups were consistent with the finding by Kraemer (1993), he suggested that the improvement in strength was caused by the increase in testosterone levels. Jones and Round (1996) proposed that increases in strength are greater than increases in muscle size during the first 6-8 weeks of strength training. Thus, an increase in arm circumference observed in treatment group could be explained by the testosterone enhancing effect of the extract. In conclusion, results obtained from this pilot study suggest that the administration of LJ100 improved fat free mass, reduce fat mass, increase muscle strength and size suggesting LJ100 might be used as an ergogenic aid. Further studies will be carried out to determine the mechanism of action at hormonal and molecular level.

    Water-soluble extract of LJ100? as a potential
    natural energizer for healthy aging in men.
    M.I.M.TAMBI1, S. OTHMAN2and J.M SAAD2

    1Specialist Reproductive Research Center, National Population & Family Development Board, Ministry of Women & Family Development, Malaysia.
    2Department of Biochemistry, Faculty of Medicine, University of Malaya, Malaysia.

    Introduction
    Malaysia has a rich source of rainforests that contain thousands of plants with potential medicinal values. One such plant is the tall shrub tree from the Simaroubaceace family, Eurycoma Longifolia (LJ100? Tongkat Ali) which is commonly found along the hilly jungle slopes of Malaysia (Burkill and Hanif, 1930). The local name of the shrub is 'Tongkat Ali' or Ali's Walking Stick' which is rather suggestive of its traditional function of sexual support for aging males. Similar trees are also found in other Asian Rainforests; however, it is traditionally known that only two species of the shrub namely E.Longifolia and E.apiculata have medicinal properties (Burkill and Hanif, 1930). The medicinal elements are only found within the roots. The root of Eurycoma Longifolia was used as a decoction by the natives of old Malaya, especially the elderly for strength and energy (Burkill and Hanif,1930), this practice remains to this day.
    Early experimental studies on animals were mainly focused on the aphrodisiac properties of LJ100. Mice treated LJ100 demonstrated higher frequency of mounting compared to the control group (Ali and Saad, 1993). Additionally, the serum testosterone of the dissected mice showed an increase of 480% compared to the placebo-controlled group (Ali and Saad, 1993). Further studies provided evidence that LJ100 produced a dose-dependent increase in mounting frequency in male rats, hence, acting as a potent stimulator of sexual arousal in the absence of feedback from genital sensation (Ang and Sim,1997). It was also shown that LJ100enhanced and maintained a high level of crossovers, mountings, intromissions, and ejaculations.

    Other studies showed that when the extract of LJ100 was injected into male mice, they showed intense physical activities and copulatory behavior (Ang and Sim, 1998). Even frail mice were observed to be active and alert. In another study, LJ100 was exposed to penile muscular tissue of male mice; results demonstrated that the muscular tissue was found to relax. Analysis on the mitochondria homogenates of the liver and penile muscle of the mice showed that the extract could enhance the respiration of mitochondria, leading to 60% increase in ATP production through oxidative phosphorylation (Khamis and Saad, 1993).

    Early clinical trials studied the effect of LJ100 on testicular tissues. The samples were incubated along with human testicular tissues taken from men who were orchidectomised as part of treatment of prostate cancer (Aminuddin et al, 1995). There was significant increase in the concentration of testosterone and its precursors. The results suggest that the LJ100 has the ability to increase the biosynthesis of androgens (Aminuddin et al, 1995).


    Androgen is the generic term for any natural or synthetic compound, usually a steroid hormone, that stimulates or controls the development and maintenance of masculine characteristics in vertebrates. This includes the activity of the accessory male sex organs and development of male secondary sex characteristics. The primary, and most well known, androgen is testosterone.

    In this study, we intend to investigate the effect of the LJ100 water-soluble extract on testosterone, dehydroepiandrosterone (DHEA) and sex hormone binding globulin (SHBG) levels in human subjects. Dehydroepiandrosterone (DHEA) is a natural steroid hormone produced from cholesterol by the adrenal glands. Dehydroepiandrosterone is structurally similar to testosterone and estrone and can be easily converted into those hormones (DHEA is a precursor for testosterone). Sex hormone binding globulins are carrier proteins that regulate the amount of unbound steroid in the blood. A decrease in SHBG is associated with an improvement in free testosterone index. Additional parameters that will be measured include Quality Of Life (QOL) via the PADAM score and Sexual Health Inventory Questionnaires (SHI-Q).

    Methodology
    In a Reproductive Research Center in Kuala Lumpur, Malaysia, 30 human volunteers were recruited in a randomized open trial. The volunteers were selected among married men whose age ranges between 31-52 years. There were no other specific criteria for the selection of volunteers. Dr. Johari M. Saad and co-workers from the Department of Biochemistry, University Malaya, Malaysia, produced LJ100 water-soluble extract of E.Longifolia root.
    Upon registration, the volunteers were asked to fill out two questionnaires: (i) a validated Sexual Health Inventory Questionnaires (SHI-Q) and (ii) the PADAM Score Questionnaires. Peripheral venous blood sample was collected from each individual to evaluate his total testosterone hormone, dehydroepiandrosterone sulphate (DHEA) and sex hormone binding globulin (SHBG) levels. Following this, each volunteer was given a supply of the encapsulated LJ100. These were to be consumed regularly for three consecutive weeks, twice daily, and two capsules per day (100 mg/day). The volunteers were requested to come back for a follow-up after week one and week three. During the follow-up sessions, they were asked to again fill out two sets of questionnaires and provide blood samples for analysis of serum testosterone, SHBG and DHEA.

    Results
    Questionnaires Analysis
    Analysis of the SHI-Questionnaire results have shown that 62% of the cases had either increased or a maximum score after consuming LJ100. Another 24% showed reduction while 14% of the cases showed no change in the score (Figure 1). This indicates that the majority of the volunteers demonstrated an increase in their sexual health satisfaction and performance. Breakdown of the SHI-Questionnaire showed subjects has an increase in sexual desire and the success at the attempts at sexual intercourse.


    Figure 1: Effect of LJ100? consumption on SHI-Q Score


    PADAM Analysis
    Analysis of the PADAM Score demonstrated that 82% of the cases showed a decrease in total score (decrease is positive effect). There is 91% improvement in the sexual PADAM score component, a 73% improvement in the physical component, and an 82% improvement of psychological component. The vasomotor score showed improvement in 50% of the subjects (Figure 2). The improvements in the first three components of the PADAM score reflects that consumption of LJ100 had resulted in an improvement of their quality of life with regards to their physical, sexual and psychological well being.

    Figure 2: Percentage of Improvement or Reduction for Various Components of the PADAM Score


    Serum Hormones analysis
    Testosterone
    Total testosterone levels were not significantly different between those raised (43%) and those declined (39%) in this study (Table 1). This gives an initial impression that LJ100 does not have any effect on steroidogenesis. Considering that almost all the volunteers have normal levels of total testosterone, the feedback system is activated to ensure the testosterone levels are within the individual needs range. In 6 volunteers whose serum total testosterone is low, there is an increase in total testosterone on first and third week as well as improvement in the Quality of Life Scores (SHI-Q and PADAM Score).
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    DHEA
    Analysis of the DHEA showed gradual increase from 26% after 1 week to 47% after 3 weeks. This suggests that LJ100may influence the DHEA production, which subsequently would be aromatized to testosterone (Figure 3).

    Figure 3: Percentage of Increment in DHEA level





    SHBG Analysis
    The results showed that SHBG levels were reduced in 36% of the cases after one week and improved to 66% after 3 weeks. This suggests that LJ100 could have an effect on the production of SHBG (Table 2).





    Free Testosterone Index Analysis (FTI)
    When the SHBG level declines, the Free Testosterone Index (FTI is calculated as a percentage of the total testosterone against SHBG) goes up. Results demonstrated that the FTI increased in 39% of the subjects after 1 week to 73% after 3 weeks (Table 3)




    Conclusion
    Increasing testosterone is the key factor in increasing sex drive. Testosterone is the most important of the male sex hormones, known as androgens, produced in the gonads. Testosterone plays a key role in the development and maturity of male sex organs. The hormone promotes secondary sex characteristics, including the appearance of facial hair, sexual desire, and sexual behavior. However, testosterone is not just a sex booster for men. Women also produce testosterone, about 5 to 10 percent the amount produced in men. In woman, this vital hormone also stimulates sex drive and produces heightened sensitivity of erogenous zones.

    In this study, the aqueous LJ100 extract has a strong potential in providing sufficient free testosterone to the body as demonstrated by the increase in the free testosterone index between weeks one and three. The high score in the Physical and Sexual Domain of PADAM and the Desire and Sexual Attempts in the SHI-Q score suggests this extract can delivery sexual health effects for both men and women.

    The results demonstrated that the circulating androgen concentration affects SHBG synthesis. The increase in DHEA levels (DHEA is a precursor to testosterone) between week 1 and week 3 resulted in elevated testosterone levels that caused a decrease in SHBG levels. It is important to note the any decrease in SHBG levels has an overall effect to increase free testosterone index as indicated in table 3. The results of this study suggest that LJ100 inhibits SHBG allowing more free testosterone to remain in the blood. This additional testosterone stems the aging process, improves energy and sexual function, and helps reduce body fat and reduces the risk factors associated with heart health.

    Since this study is just an exploratory study to look into the marketing potential of LJ100, the volunteers were asked about personal feedbacks with regard to the extract. The following responses were received:
    48% felt that they are feeling healthy, not easily tired, feeling active and energized.
    40% felt easily aroused, increase sexual desire and maintained an erection longer.
    16% felt their joints and backache are feeling better.
    24% felt warm and easily sweat (sign of better circulation)
    8% experience better sleep.
    8% felt an improvement in their memory.
    20% felt their appetite has improved and their bowel movements are better than before.

    References
    1.Burkill, IH and Hanif, M; (1930) Malay Village Medicine, The Garden Bulletin Strait Settlements.
    2.Ali, JM and Saad, JM (1993); Biochemical effect of Eurycoma Longifolia Jack on the sexual behavior, fertility, sex hormone and glycolysis. Dissertation Paper for Bachelor of Science, Department of Biochemistry, University of Malaya
    3.Ang, HH and Sim,MK (1997); Effect of Eurycoma Longifolia Jack on sexual behavior of male rats. Archives of Pharmacal Research (Seoul),20(5),656-58
    4.Ang, HH and Sim,MK (1998)[1]; Eurycoma Longifolia Jack and orientation in sexually experiences male rats. Biol and Pharmaceutical Bulletin 21(2);153-55
    5.Ang, HH and Sim,MK (1998)[2]; Eurycoma Longifolia Jack increases sexual motivation in sexually naive male rats. Archives of Pharmacal Research (Seoul),21(6),778-81
    6.Khamis, ZM and Saad, JM (1993); Dissertation Paper for Bachelor of Science, Department of Biochemistry, University of Malaya.
    7.Aminuddin, N; Saad, JM; Hadi, AH and Abdullah, R (1995); The effect of Eurycoma Longifolia extracts on androgen synthesis.


    LJ100? Saliva Testosterone Test

    Saliva Testosterone Test of 9 Individuals 26-52 years of age
    Dosage 2x2 (50mg/capsules) morning & evening for 10 days
    Normal range for athlete 800 = 150ng/dl of blood



    Volunteers 1-5 are athletes - data are an average of 3 different studies at different times
    Volunteers 6-9 do not exercise on a regular basis

    Conclusion
    The results demonstrate that 100 mg per day of LJ100 caused an increase in bioavailability testosterone within 10 days. Furthermore, all subjects (athletes and non-athletes) showed a positive increase in testosterone suggesting that LJ100 causes an increase in the free testosterone index.

    Another:

    Effect of LJ100 Tongkat Ali on Anabolic Balance During Endurance Exercise

    Talbott S, Talbott J, Negrete J, Jones M, Nichols M, and Roza J. Effect of Eurycoma longifolia Extract on Anabolic Balance During Endurance Exercise. SupplementWatch, Inc. Draper, UT, 84020 USA and Source One Global Partners, Chicago, IL 60611 USA. smtalbott@supplementwatch.com

    Eurycoma longifolia, commonly known as ?Tongkat Ali? or ?Longjack,? is often touted as a testosterone ?booster? and marketed to athletes as a training aid and performance enhancer. Rodent studies have shown oral delivery of Eurycoma extract to improve sexual performance and increase serum testosterone levels. Open-label human trials have suggested that Eurycoma extract may help prevent age-associated androgen deficiency, improve sexual function, and increase psychological parameters such as mood, energy, and sense of well-being. The purpose of this study was to determine the effects of Eurycoma longifolia on testosterone and cortisol levels during intense endurance exercise. We used a water-soluble extract of Eurycoma longifolia (E) standardized to 22% eurypeptides and 40% glycosaponins. Male subjects (N=30) were recruited from a 24-hour mountain biking event and asked to provide a saliva sample before and after each lap for measurement of cortisol and testosterone by enzyme immunoassay (Salimetrics, State College, PA). Subjects completed 4 laps (14.91 miles/lap) and provided 8 saliva samples over a 24h period. Subjects consumed 100mg of E or a look-alike placebo (P) approximately 30 minutes prior to endurance exercise. Cortisol levels were 32.3% lower in E compared to P (0.552+0.665 versus 0.816+0.775 ug/dL, P < 0.05). Testosterone levels were 16.4% higher in E compared to P (86.72+40.90 versus 72.47+33.77 pg/mL, P < 0.05). These results suggest that Eurycoma longifolia extract may help to maintain normal levels of cortisol (low) and testosterone (high) and thus promote an overall ?anabolic? hormonal state (versus a ?catabolic? state characterized by elevated cortisol and suppressed testosterone) during intense endurance exercise.
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    *PUBMED* Study showing Tribulus INCREASES TESTOSTERONE

    The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction - an evaluation using primates, rabbit and rat.

    Gauthaman K, Ganesan AP.
    Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, 119074 Singapore.

    Hormonal effects of Tribulus terrestris (TT) were evaluated in primates, rabbit and rat to identify its usefulness in the management of erectile dysfunction (ED). TT extract was administered intravenously, as a bolus dose of 7.5, 15 and 30mg/kg, in primates for acute study. Rabbits and normal rats were treated with 2.5, 5 and 10mg/kg of TT extract orally for 8 weeks, for chronic study. In addition, castrated rats were treated either with testosterone cypionate (10mg/kg, subcutaneously; biweekly for 8 weeks) or TT orally (5mg/kg daily for 8 weeks). Blood samples were analyzed for testosterone (T), dihydrotestosterone (DHT) and dehydroepiandrosterone sulphate (DHEAS) levels using radioimmunoassay. In primates, the increases in T (52%), DHT (31%) and DHEAS (29%) at 7.5mg/kg were statistically significant. In rabbits, both T and DHT were increased compared to control, however, only the increases in DHT (by 30% and 32% at 5 and 10mg/kg) were statistically significant. In castrated rats, increases in T levels by 51% and 25% were observed with T and TT extract respectively that were statistically significant. TT increases some of the sex hormones, possibly due to the presence of protodioscin in the extract. TT may be useful in mild to moderate cases of ED.

    *PUBMED* Another

    Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats.Gauthaman K, Adaikan PG, Prasad RN.

    Department of Obstetrics and Gynaecology, National University Hospital, National University of Singapore, Singapore 119704, Singapore.

    Tribulus terrestris (TT) has long been used in the traditional Chinese and Indian systems of medicine for the treatment of various ailments and is popularly claimed to improve sexual functions in man. Sexual behaviour and intracavernous pressure (ICP) were studied in both normal and castrated rats to further understand the role of TT containing protodioscin (PTN) as an aphrodisiac. Adult Sprague-Dawley rats were divided into five groups of 8 each that included distilled water treated (normal and castrated), testosterone treated (normal and castrated, 10 mg/kg body weight, subcutaneously, bi-weekly) and TT treated (castrated, 5 mg/kg body weight, orally once daily). Decreases in body weight, prostate weight and ICP were observed among the castrated groups of rats compared to the intact group. There was an overall reduction in the sexual behaviour parameters in the castrated groups of rats as reflected by decrease in mount and intromission frequencies (MF and IF) and increase in mount, intromission, ejaculation latencies (ML, IL, EL) as well as post-ejaculatory interval (PEI). Compared to the castrated control, treatment of castrated rats (with either testosterone or TT extract) showed increase in prostate weight and ICP that were statistically significant. There was also a mild to moderate improvement of the sexual behaviour parameters as evidenced by increase in MF and IF; decrease in ML, IL and PEI. These results were statistically significant. It is concluded that TT extract appears to possess aphrodisiac activity probably due to androgen increasing property of TT (observed in our earlier study on primates).



    TRIBESTAN EFFECT ON THE CONCENTRATION OF SOME HORMONES IN THE SERUM OF HEALTHY SUBJECTS

    S. Milanov, A. Maleeva, M. Taskov

    RIRR - Radioisotope and Radioimmunological Laboratory, Sofia

    Chemical Pharmaceutical Research Institute,
    Sofia, Bulgaria

    SUMMARY

    Tribestan effect has been studied on the serum concentration of hypophyseal hormones, of ACTH, STH, LH, FSH, adrenal hormone aldosterone and cortisol and sex hormones - testosterone and estradiol. The experiments have been carried out on 8 males and 8 females, aged 28 - 45 years of age. The product was perorally administered in a single dose of 250 mg, three times daily for 5 days. Serum samples were withdrawn at 8 a.m. and 12 a.m., prior to and post treatment. The product has been established not to change essentially the concentrations of adrenal hormones and of ACTH. The hypophyseal-gonadal axis however has significantly been affected in the females with predominantly increased concentration of FSH and estradiol and in the males - mainly of LH and the testosterone. The mechanism of that action is presumed to be complicated and realized both by direct effect on gonadal apparatus and by the tropic hormones.
    The probable established changes in the concentration of the hormones studied do not get out of the frames of the physiological limits.

    The lyophilized extract of Tribulus terrestris, introduced in veterinary practice as TB-68, has pronounced sex-stimulating function. The initial studies of this product showed that it stimulates the spermatogenesis of albino rats (Vankov S., et al., 1973) and enhanced the ovulation of female rats (Vankov S. et al. 1973). Zarkova S. (1976) has also established in rats an increased number of spermatogonia, spermatocytes as well as increase of neutral mucopolysaccharides in seminiferous tubules of the testes. Gendzhev Z. and S. Zarkova, in other experiments (1978) proved the increase of spermatic reserve in the epididymis of rats.

    With the view to the need of human medicine of a product stimulating sexual function, Tribestan was formulated on the base of the indicated phytochemical product. It contains saponins of furostanol type (Tomova M. et al., 1978). The first studies of Tribestan confirmed its high sex-stimulating activity in experimental animals (Zarkova S., 1981). Later, the clinical studies established a similar stimulating effect in humans as well (Protich M. at al. 1981). The present study was carried out with a view to throwing light on some aspects of the mechanism of that action of Tribestan, aiming at attaining an effect from the product on the serum concentration of some hypophyseal, sexual and adrenal hormones.

    MATERIALS AND METHODS

    The experiments were performed on 16 subjects (8 females and 8 males), aged 28-45. All subjects were in good health, without any complaints and good capacity for work. The following schedule was used:
    1. The basic levels of hypophysiotropic hormones (ACTH, STH, LH, FSH), of sexual hormones (testosterone and estradiol) and of adrenal hormones (aldosterone and cortisol) were determined. They were determined twice, at 8 a.m. and 12 p.m. - one day prior to Tribestan treatment.
    2. The treatment with the product was initiated on the following day, which was periodically administered, 250 mg, three times daily for 5 days.
    3. After the termination of Tribestan treatment (day sixth after the initiation of the experiment), blood was again withdrawn (at the same hour - 8:00 a.m. and 12 p.m.) for the determination of the concentration of the indicated hormones.

    The work proceeded in the following way: after centrifugation of 6 - 8 ml blood, the serum obtained was frozen at 20?C till the day of the determination of hormonal concentration. The determination was performed by radioimmune tests. LH and FSH were determined by the modified method of Midgley A.R., (1967), making use of some kits of Biodata company, Italy and ACTH and STH - according to the method of Berson S.A. and R. S. Yalow (1963). Testosterone was evaluated by the method of William R. H. (1968), and of estradiol by Orezyk G.P. et al. (1974), making use of kits of Sorin Company, Belgium for both hormones. The adrenal hormones cortisol and aldosterone were also determined by kits of that company, making use of Vescei P. (1974) and of William G and R. Hunderwood (1974).

    The obtained results were statistically processed by variation analysis, by Student - t test.
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    RESULTS AND DISCUSSION

    As could be seen from Table 1, LH level in the males was elevated with a high significance after the treatment (p < 0.001). The changes affected both samples to the same rate (at 8 a.m. and 12 p.m.). FSH concentration was not affected under the same conditions. The other two hypophyseal hormones, ACTH and STH were not changed.

    An insignificant tendency to elevation was observed in STH level (mean values - 2.9 prior to and 3.2 mg/ml post treatment) in some of the cases. The level of sex hormones was strongly affected. Thus testosterone concentration was three-fold (2) increased and that of estradiol - about 1.5 times (Table 1).

    Table 1
    Hormone Prior to Tribestan
    Post Tribestan

    8 a.m.
    12 p.m.
    8 a.m.
    12 p.m.

    LH, mIU/ml X 13.0 14.38(1) 37.25 24.75
    SX 0.64 0.73 1.01 0.79
    Pt 0.001 0.001
    FSH, mIU/ml X 13.38 13.50 13.38 11.38
    SX 0.35 0.28 0.35 0.36
    Pt >0.5 >0.5
    Testosterone, ng % X 628 610 882 845
    SX 48 46 35 32
    Pt <0.001 <0.001
    Estradiol pg/ml X 79 76 133 137.5
    SX 3.46 2.24 6.72 5.86
    Pt <0.001 <0.001

    LH concentration was also increased in females under Tribestan effect. What impressed was that the significance was lower than the first sample. The greatest discrepancy, as compared with the results of the males, was the sharp stimulation of FSH. A strong effect was observed there, which could be explained by blood withdrawal during the early phase of the menstrual cycle, the so-called follicular phase. Estradiol was also strongly affected (Pt < 0.001), whereas testosterone in the females during the early hours of the day was less affected (Table 2).

    Table 2
    Hormone
    Prior to Tribestan
    Post Tribestan

    8 a.m.
    12 p.m.
    8 a.m.
    12 p.m.

    LH, mIU/ml X 15.25 13.50 17.13 16.88
    SX 0.64 0.87 0.73 0.35
    Pt 0.02 0.001
    FSH, mIU/ml X 11.00 11.88 17.75 15.25
    SX 0.13 0.09 0.71 0.38
    Pt 0.001 0.001
    Estradiol mIU/ml X 72.13 59.38 77.13 87.50
    SX 6.02 5.73 5.47 3.24
    Pt 0.5 0.001

    The level of adrenal hormone was identically affected both in males and females (Table 3). A significant increase of the concentration was also established though that effect had a relatively low significance (p < 0.05). At the same time, cortisol level was no changed (Table 3).

    Table 3
    Aldosterone Cortisol
    Prior to Post Prior to Post
    X 11.59 13.77 8.63 8.63
    S 2.52 3.48 2.20 1.92
    SX 0.63 0.87 0.55 0.48
    Pt 0.05 0.05

    The results obtained provided grounds to admit that Tribestan had a pronounced stimulating effect on the secretion of some hormones. The effect on the hormones along the hypophyseal-gonadal axis was particularly well manifested. The effect was manifested both at hypophyseal and gonadal level. Some sexual discrepancies were also established. Thus, FSH was mainly affected in the females. The presence of that hormone is exceptionally important during the follicular phase for the development of the follicle. When its development is stimulated, its secretory ability is also intensified and hence - estradiol level is elevated. Lutenizing hormone is more strongly influenced in the male, which on its part stimulates the secretion of testosterone.

    ACTH and cortisol were not changed suggesting that they were not significantly involved in the realization of Tribestan effects. The tendency of stimulation of STH and aldosterone explained the activation of the anabolic processes in the body and general stimulating action of the product. The absence of effect on the level of cortisol showed however that the general tonic action was very strongly manifested.

    It should be stressed that the level of the hormones studied did not go out beyond the physiological frames i.e. it did not disturb the physiological mechanisms of hormonal regulation.

    References

    Vankov S., S. Zarkova, Z. Gendzhev, M. Tomova - Effect of TB-68 on the spermatogenesis in albino rats. Proceeding of the Third National Conference of Pharmacology and Clinics of New Bulgarian Drugs, Sofia, November 14-16, 1973, v.2, 161-163.
    Vankov S., S. Zarkova, M. Tomova - TB-68 effect on ovulation of albino rats. Proceedings of Third National Conference of Pharmacology and Clinics of New Bulgarian Drugs, Sofia, November 14-16, 1973, v.2, 165-167.
    Gendzhev Z., S. Zarkova - Effect of the phyto-pharmaceutical TB-68 on the number of spermatozoa in epididymis of rat. Med. Archive, 1978, N I, 113-118.
    Dimova P., M. Taskov - Comparative enzyme-histological studies of some phyto-products. MBI (at the printer's), 1981.
    Zarkova S. - Morphological and histological changes in testes of rat under the effect of TB-68, Med. Archive, 1976, N 4, 49-53.
    Protich M., D. Zvetanov, V. Nalbanski, R.Stanislavov, M.Kazarova - Clinical trial of Tribestan on infertile males, MBI (at the printer's).
    Tomova M., V. Gyulemetova, S. Zarkova - Author's certificate N 77584 A 61 K 35/1978.
    Berson S.A., R. S. Yalow - Immunoassay of protein hormones, The Hormones, Vol. V, Acad. Press., New York, 1963.
    Midgley A.R. - Radioimmunoassay for Human, J. Clin. Endocr., 1967, 27, 295.
    Orezyk, Gaylo P., Burton v. Caldwell, Harold H. Behrmaan - Methods of Hormone Radioimmunoassay - Ed. B. Jaffe, H. Berhmaan, A6. Press, NJ, London, 1974, 333-343.
    Vescei P. - Glicocorticoids: Cortisol Corticosterone - Methods of Hormone Radioimmunoassay; Ed. B. Jaffe and H. Behrmaan, Ac. Press, NJ, London 393-412.
    William R.H. - Textbook of Endocrinology 4th Edit. Saunder, Philadelphia, 1968.
    Williams Gordon H., Richard H. Hunderwood - Methods of Hormon Radioimmunoassay; Ed. B. Jaffe and H. Behrmaan, Ac. Press, NJ, London, 1974, 371-390.
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    Longjack Review By Dr. Simon Chua, Department of Pharmaceutical Sciences, Wayne State University

    In a preliminary study at the Reproductive Research Center in Kuala Lumpur , Malaysia , 30 married men, aged31-52 years old, took Longjack regularly for three consecutive weeks. Interviews indicated that 48% felt healthy, active and energized, and 40%felt increased sexual desire. The subjects showed an increased free testosterone index. Increased testosterone levels help improve libido, energy and muscle mass for bodybuilding. Their levels of DHEA, another critical steroid hormone, gradually increased from 26%after one week to 47% after three weeks. Most subjects demonstrated increased satisfaction in their sexual health and performance.

    In addition, animal studies have shown that Longjack may increase testosterone availability as well as libido in mice. (Source: Article from the book Molecular Biology of the Cell, Published on: Sep 1, 2002)

    Longjack Review By Dr. James Stoxen, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago

    The production of testosterone (the principal male-determining sex hormone) falls progressively from age 40. Experts call this ?partial androgen deficiency of the aging male.? Androgens are steroid hormones with specific effects on tissue growth (muscle, fat, skin, hair and others) and brain function. They play important roles in men and women, but are produced in much larger quantities in men. In men after puberty, the testicles produce most androgens, mainly as testosterone. It has been suggested that Longjack may block negative feedback to the hypo-thalamus and pituitary glands, so the body continues to produce testosterone at elevated levels. (Source: Chapter 39-a from the book Eureka Bioscience Collection , Published on: 3 rd Oct, 2003)

    Longjack Review By Ang HH, Ikeda S, Gan EK. School of Pharmaceutical Sciences,
    University Science Malaysia, Minden, Penang, Malaysia

    The butanol, methanol, water and chloroform extracts of the roots of Eurycoma longifolia Jack were studied using various tests of potency of treated male rats. The results showed that E. longifolia produced a dose-dependent, recurrent and significant increase in the episodes of penile reflexes as evidenced by increases in quick flips, long flips and erections of the treated male rats during the 30 min observation period. These results provide further evidence that E. longifolia increases the aphrodisiac potency activity in treated animals. (Source: Journal index for medicine? 2001 John Wiley & Sons, Ltd, Published on: Dec 2005.)
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    Originally Posted by SupremeSE View Post
    Discussion/ Conclusion
    In this study, although the testosterone level was not measured during the test period, an increased in fat in treatment group may be linked to the rise in steroidal hormones in the body. The percentage of body fat appeared to increase in both groups, this finding is in agreement with a study by Brodsky (1996).
    O HAI, Look Who is back
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    Originally Posted by Taman25thc02 View Post
    O HAI, Look Who is back
    Back and BETTER than ever
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    ٩(●̮̮̃•̃)۶ ٩(●̮̮̃•̃)۶ iForce Dave's Avatar
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    oh boy


    *popcorn-eating smiley animation*
    keep it simple, keep it basic, keep it heavy
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    Originally Posted by SupremeSE View Post
    Back and BETTER than ever
    Hey..you delivered the Omni as promised..I got no problems with you anymore...so long as that ECDY is really 99% pure.
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    Originally Posted by SupremeSE View Post
    Back and REDDER than ever
    fixed
    *~=Gator Nation=~*

    If I was in a room and someone yelled "Hey Bitches!" I would never turn around because I just don't fit the description.
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    Originally Posted by Taman25thc02 View Post
    Hey..you delivered the Omni as promised..I got no problems with you anymore...so long as that ECDY is really 99% pure.
    I told you my man, Supreme Sports Enhancements is GOLDEN.

    We are also now releasing a NEW Omnibolic product that will be released the SAME DAY as the new EBOL. Competition is GOOD.
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    Ross, I'll give you this, your copy and paste skills rival the best in the copy and paste business.

    Take note. The pub meb results were on rats and rabbits, and there was NO increase in test levels.

    The crap ad posing as a study out of Asia is just that. An ad.

    The RIGHT way to test a product would be the way Ergopharm did 6OXO. Why don't you do your own test the same way?

    Real men. Real blood work. Real results. A base line was established. An end result was measured.

    Why don't you do that ross? Just prove us wrong. Your a big time supplement company. Do a double blind, placebo control study. That would prove EVERYTHING. Use a real university here in the states. Use your product.

    You offered me a money back guaranty 6 months ago. I still want it. I have sent you my order #. Are you a man of your word or not? Are you honest?
    I told you your product didn't work for me, and I spent money to see if it would only because you PROMISED me my money back if it didn't.

    You create a PR DISASTER every where you go. Clean up the messes you've made.

    There is a reason your universally disliked. You LIE, CHEAT, and STEAL.

    You can start repairing your insidious rep right here, right now, or you can continue to receive the grief you so well deserve.

    Personally, I'm sure you will bitch out.
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  25. #25
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    Originally Posted by SupremeSE View Post
    We are also now releasing a NEW Omnibolic product that will be released the SAME DAY as the new EBOL. Competition is GOOD.
    I just got my bottle last week and now its already about to be obsolete?
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    Originally Posted by Taman25thc02 View Post
    I just got my bottle last week and now its already about to be obsolete?
    Is EBOL obsolete? No, and until they release their NEW version, people will continue to use the current version.

    The NEW version of Omnibolic has not even been completely determined, but research is underway.

    ENJOY!!
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    Originally Posted by jeff3kids View Post
    Ross, I'll give you this, your copy and paste skills rival the best in the copy and paste business.

    Take note. The pub meb results were on rats and rabbits, and there was NO increase in test levels.

    The crap ad posing as a study out of Asia is just that. An ad.

    The RIGHT way to test a product would be the way Ergopharm did 6OXO. Why don't you do your own test the same way?

    Real men. Real blood work. Real results. A base line was established. An end result was measured.

    Why don't you do that ross? Just prove us wrong. Your a big time supplement company. Do a double blind, placebo control study. That would prove EVERYTHING. Use a real university here in the states. Use your product.

    You offered me a money back guaranty 6 months ago. I still want it. I have sent you my order #. Are you a man of your word or not? Are you honest?
    I told you your product didn't work for me, and I spent money to see if it would only because you PROMISED me my money back if it didn't.

    You create a PR DISASTER every where you go. Clean up the messes you've made.

    There is a reason your universally disliked. You LIE, CHEAT, and STEAL.

    You can start repairing your insidious rep right here, right now, or you can continue to receive the grief you so well deserve.

    Personally, I'm sure you will bitch out.
    Candidate for post of the year
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  28. #28
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    oh boy supp drama!

    get yo popcrn rdy
    Last edited by muscleman420; 07-03-2008 at 11:03 AM.
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    Originally Posted by jeff3kids View Post
    Ross, I'll give you this, your copy and paste skills rival the best in the copy and paste business.

    Take note. The pub meb results were on rats and rabbits, and there was NO increase in test levels.

    The crap ad posing as a study out of Asia is just that. An ad.

    The RIGHT way to test a product would be the way Ergopharm did 6OXO. Why don't you do your own test the same way?

    Real men. Real blood work. Real results. A base line was established. An end result was measured.

    Why don't you do that ross? Just prove us wrong. Your a big time supplement company. Do a double blind, placebo control study. That would prove EVERYTHING. Use a real university here in the states. Use your product.

    You offered me a money back guaranty 6 months ago. I still want it. I have sent you my order #. Are you a man of your word or not? Are you honest?
    I told you your product didn't work for me, and I spent money to see if it would only because you PROMISED me my money back if it didn't.

    You create a PR DISASTER every where you go. Clean up the messes you've made.

    There is a reason your universally disliked. You LIE, CHEAT, and STEAL.

    You can start repairing your insidious rep right here, right now, or you can continue to receive the grief you so well deserve.

    Personally, I'm sure you will bitch out.
    It has already been done, LJ100* is PATENTED and owned by HP-Ingredients, I simply signed a trademark agreement to include LJ100 in AndroGenerator.

    The PATENT was granted because of the UNIVERSITY studies I posted above. LJ100 is scientifically proven to increase T.
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  30. #30
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    Excuse me captain cut and paste, but your study was done by chemical pharmaceutical research institute,
    Sofia, Bulgariaudy. They just happen to sell your ingredient.

    Again, put the debate to rest and have your product double blind, placebo controlled tested. Your a big time supplement company with your own marketing department and everything, just do it and shut us up.

    Ergopharm did it, why wont you? There are at least 20 other companies here that have there products tested. Add yours to the list.

    And again, be a man of your word and refund my money.

    Originally Posted by SupremeSE View Post
    It has already been done, LJ100* is PATENTED and owned by HP-Ingredients, I simply signed a trademark agreement to include LJ100 in AndroGenerator.

    The PATENT was granted because of the UNIVERSITY studies I posted above. LJ100 is scientifically proven to increase T.
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