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10-23-2009, 01:39 PM
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#1
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MICROBIOLOGY ASSAY!! I need HELP (Substrate COncenrtration vs OD)
Sample work problem.
Substrate concentration (mM)
( Tubes were made with the following concentrations of SUbstrate...(any substrate)
In mM 10 20 30 40 50 60 70 80 90
time0 0.005 0.005 0.004 0.005 0.005 0.00 0.004 0.005 0.295
48hrs 0.095 0.17 0.23 0.26 0.275 0.28 0.295 0.295 0.296
QUESTION TO PROBLEM IS:
You have a sample of some food that when this bacterium grows on the extract it reaches an OD of 0.205 after 48hrs and shows not further increase. When started it had an OD of 0.005. How many moles of substrate did the extract contain?
I have managed to do a graph that looks like this
OD Reading l
(yaxis) l
l
l an upside down parabola goes here!!!
l
l
l________________
(x-axis) [S] (Substrate concentration( the difference from time 0 to after 48hrs of incubation)
I added and subtracted the difference in substrate concentration, from beginning to ending
These are the results I got
from the 10mM all the way through 90mM
=
In mM for tube with 10 mM.009
In mM for tube with 20 mM.165
In mM for tube with 30 mM = .226
In mM for tube with 40mM =.255
In mM for tube with 50 mM =.270
In mM for tube with 60 mM = .28
In mM for tube with 70 mM =.291
In mM for tube with 80 mM = .29
In mM for tube with 90 mM =.001
I plotted these on the x axis, against the OD reading (Data given at the top, for each tube)
I have no idea how to answer the question it is asking...!
What should I do,how do I solve this problem??????????
PLEASE Help Me!!!
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Last edited by CarDent; 10-23-2009 at 02:14 PM.
Reason: Error
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10-23-2009, 01:42 PM
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#2
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wtf is this ****
assay
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boobs ftw
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10-23-2009, 01:43 PM
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#3
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Quote:
Originally Posted by kalmamd
wtf is this ****
assay
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It's a bio assay
it's what microbiologists DO
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"El Arte es una mentira que nos hace realizar la verdad"
Piccasssssssso
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10-23-2009, 01:44 PM
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#4
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misc broscientist grad.
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Well I got my broscience degree in physics, so i'm not help here.
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"It's a big enough umbrella, but its always me that ends up getting wet"- The Police
Workout Log (reps if you talk in it): http://forum.bodybuilding.com/showthread.php?t=118623401
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10-23-2009, 01:52 PM
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#5
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The answer is 3
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10-23-2009, 01:55 PM
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#6
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lol, this was the only thing i could not do in micro....passed w/ a b tho,
GOOD LUCK BRO!
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10-23-2009, 01:59 PM
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#7
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The One and Only
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We didnt learn how to do this in first year microbio. sorry baaaaahhhh
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Super Buff Awesome
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10-23-2009, 02:02 PM
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#8
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Registered User
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thx fort readin
BTW...
I am a female
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"El Arte es una mentira que nos hace realizar la verdad"
Piccasssssssso
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10-23-2009, 02:05 PM
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#9
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Quote:
Originally Posted by lifeguard_man
We didnt learn how to do this in first year microbio. sorry baaaaahhhh
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What's the name of your course? I'm in Microbiology and Immunology, I might be able to help.
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10-23-2009, 02:13 PM
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#10
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Quote:
Originally Posted by WhiteKMaro
What's the name of your course? I'm in Microbiology and Immunology, I might be able to help.
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I go to a Cal State University...
course number
BIOLOGY 417
ALSO, i just aditted a little, mistake in the
In mM for tube with 10 mM.,, it was supposed to be consecutive numbering
10, 20, 30, 40, 50.. and so on... til 90
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"El Arte es una mentira que nos hace realizar la verdad"
Piccasssssssso
Last edited by CarDent; 10-23-2009 at 02:15 PM.
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10-23-2009, 02:17 PM
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#11
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Quote:
Originally Posted by Yeahbudday
OP what does OD stand for?
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It is the absorbance, or reading of a machine ( Machine is called and spectrophotometer)
It is used to read how much light was deflected or absorbed by the machine, this reading can tell you how much or an organism you have in a sample, which is inside a cuvette, a special squared tube
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"El Arte es una mentira que nos hace realizar la verdad"
Piccasssssssso
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10-23-2009, 02:19 PM
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#12
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Quote:
Originally Posted by CarDent
thx fort readin
BTW...
I am a female
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How is that relevant?
An attention whore in the making?
negs
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10-23-2009, 02:22 PM
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#13
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Quote:
Originally Posted by cs21
How is that relevant?
An attention whore in the making?
negs
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attention whore?
look who is talking... looking liek you are the one looking for attention... calling me an attention whore, sounds like you want me to reply to your messages...
You are such a GOQ
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"El Arte es una mentira que nos hace realizar la verdad"
Piccasssssssso
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10-23-2009, 02:23 PM
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#14
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Well you can determine the molarity of a solution by its absorbance on a spectrophotometer no? I don't really know how to do it I didn't do Biol4s I'm only at Biol200 lol and MIMM212 and 211 ...
but try to look at this website
http://www.chm.davidson.edu/ronutt/c...ro/Spectro.htm
sorry. good luck!
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10-23-2009, 02:27 PM
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#15
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Quote:
Originally Posted by WhiteKMaro
Well you can determine the molarity of a solution by its absorbance on a spectrophotometer no? I don't really know how to do it I didn't do Biol4s I'm only at Biol200 lol and MIMM212 and 211 ...
but try to look at this website
http://www.chm.davidson.edu/ronutt/c...ro/Spectro.htm
sorry. good luck! |
THANKs, I'll try to see if it helps.
Thank you
__________________
"El Arte es una mentira que nos hace realizar la verdad"
Piccasssssssso
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