High-Voltage Body Octane - Ingredient/Dosing Research

[dwm230000]

What's In It?
Supplement Facts:

Serving Size: 1 Scoop (11.5g)
Servings Per Container: 50

Amount Per Serving:

Vitamin B6 5mg
Vitamin B12 15mcg
Pantothenic Acid 10mg
Niacin 25mg
Folic Acid 400mcg

Citrulline Malate 3.5g
Glucuronolactone 1g
Beta-Alanine 1.6g
L-Aspartic Acid 750mg
L-Carnitine L-Tartrate 1g
Histidine HCl 250mg

Other Ingredients:
Maiz starch, citric acid, calcium phosphate, silicon dioxide, natural and artificial flavor, maltodextrin, sodium chloride, sucralose.

Directions: On training days mix one level scoop in 12 oz. of water or with a sports drink twice daily. Take one serving approximately 30 minutes prior to exercise and another serving first thing in the morning or in the afternoon depending on what time you train. Each serving should be seperated by a minimum of 5 hours. On rest days take one serving upon waking and one serving before sleep

This thread makes some of the research on the major ingredients and doses of High-Voltage Body Octane easily accessible to readers. I’ve included the abstracts along with some of the full texts(whatever I could find that was available for free) to a number of studies on these ingredients. I copy/pasted some of them for emphasis, but even those studies are still linked back in the titles. I tried to include mostly studies that were done in humans, as I know that is what people are most interested in.


Citrulline Malate


Citrulline/malate promotes aerobic energy production in human exercising muscle - Full Text (http://bjsm.bmjjournals.com/cgi/content/full/36/4/282)


Citrulline/malate promotes aerobic energy production in human exercising muscle - Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12145119&dopt=Citation)

Bendahan D, Mattei JP, Ghattas B, Confort-Gouny S, Le Guern ME, Cozzone PJ.

Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculte de Medecine de la Timone, 27 Boulevard Jean Moulin, 13005 Marseille, France.

BACKGROUND: Previous studies have shown an antiasthenic effect of citrulline/malate (CM) but the mechanism of action at the muscular level remains unknown. OBJECTIVE: To investigate the effects of CM supplementation on muscle energetics. METHODS: Eighteen men complaining of fatigue but with no documented disease were included in the study. A rest-exercise (finger flexions)-recovery protocol was performed twice before (D-7 and D0), three times during (D3, D8, D15), and once after (D22) 15 days of oral supplementation with 6 g/day CM. Metabolism of the flexor digitorum superficialis was analysed by (31)P magnetic resonance spectroscopy at 4.7 T. RESULTS: Metabolic variables measured twice before CM ingestion showed no differences, indicating good reproducibility of measurements and no learning effect from repeating the exercise protocol. CM ingestion resulted in a significant reduction in the sensation of fatigue, a 34% increase in the rate of oxidative ATP production during exercise, and a 20% increase in the rate of phosphocreatine recovery after exercise, indicating a larger contribution of oxidative ATP synthesis to energy production. Considering subjects individually and variables characterising aerobic function, extrema were measured after either eight or 15 days of treatment, indicating chronological heterogeneity of treatment induced changes. One way analysis of variance confirmed improved aerobic function, which may be the result of an enhanced malate supply activating ATP production from the tricarboxylic acid cycle through anaplerotic reactions. CONCLUSION: The changes in muscle metabolism produced by CM treatment indicate that CM may promote aerobic energy production.

Daily dose of Citrulline Malate used in study: 6 grams
Daily dose of Citrulline Malate delivered by Body Octane: 7 grams



Activity of citrulline malate on acid-base balance and blood ammonia and amino acid levels. Study in the animal and in man – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1930358&dopt=Citation)



Citrulline malate effects on the aerobic-anaerobic threshold and in post-exercise blood lactate recovery (http://coachsci.sdsu.edu/csa/vol53/janeira.htm)





L-Carnitine L-Tartrate


L-Carnitine L-tartrate supplementation favorably affects markers of recovery from exercise stress - Full Text (http://ajpendo.physiology.org/cgi/content/full/282/2/E474)

L-Carnitine L-tartrate supplementation favorably affects markers of recovery from exercise stress - Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11788381)






The effects of L-carnitine L-tartrate supplementation on hormonal responses to resistance exercise and recovery – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12930169)

Kraemer WJ, Volek JS, French DN, Rubin MR, Sharman MJ, Gomez AL,
Ratamess NA, Newton RU, Jemiolo B, Craig BW, Hakkinen K.

Human Performance Laboratory, Department of Kinesiology, University of Connecticut, Storrs, CT 06269, USA. kraemer@uconnvm.uconn.edu
The purpose of this investigation was to examine the influence of L-carnitine L-tartrate (LCLT) supplementation using a balanced, cross-over, placebo-controlled research design on the anabolic hormone response (i.e., testosterone [T], insulin-like growth factor-I, insulin-like growth factor-binding protein-3 [IGFBP-3], and immunofunctional and immunoreactive growth hormone [GHif and GHir]) to acute resistance exercise. Ten healthy, recreationally weight-trained men (mean +/- SD age 23.7 +/- 2.3 years, weight 78.7 +/- 8.5 kg, and height 179.2 +/- 4.6 cm) volunteered and were matched, and after 3 weeks of supplementation (2 g LCLT per day), fasting morning blood samples were obtained on six consecutive days (D1-D6). Subjects performed a squat protocol (5 sets of 15-20 repetitions) on D2. During the squat protocol, blood samples were obtained before exercise and 0, 15, 30, 120, and 180 minutes postexercise. After a 1-week washout period, subjects consumed the other supplement for a 3-week period, and the same experimental protocol was repeated using the exact same procedures. Expected exercise-induced increases in all of the hormones were observed for GHir, GHif, IGFBP-3, and T. Over the recovery period, LCLT reduced the amount of exercise-induced muscle tissue damage, which was assessed via magnetic resonance imaging scans of the thigh. LCLT supplementation significantly (p < 0.05) increased IGFBP-3 concentrations prior to and at 30, 120, and 180 minutes after acute exercise. No other direct effects of LCLT supplementation were observed on the absolute concentrations of the hormones examined, but with more undamaged tissue, a greater number of intact receptors would be available for hormonal interactions. These data support the use of LCLT as a recovery supplement for hypoxic exercise and lend further insights into the hormonal mechanisms that may help to mediate quicker recovery.

Daily dose of LCLT used in study: 2 grams
Daily dose of LCLT delivered by Body Octane: 2 grams



Androgenic responses to resistance exercise: effects of feeding and L-carnitine – Full Text (http://forum.bodybuilding.com/attachment.php?attachmentid=318538&d=1153286199) Thanks for posting this Des :)

Androgenic responses to resistance exercise: effects of feeding and L-carnitine – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16826026&dopt=Citation)

[dwm230000]

Beta-Alanine and Carnosine


EFFECT OF COMBINED BETA-ALANINE AND CREATINE MONOHYDRATE SUPPLEMENTATION ON EXERCISE PERFORMANCE ( http://www.acsm-msse.org/pt/re/msse/fulltext.00005768-200305001-01206.htm;jsessionid=G71TgyXGNZ2fg1bncCyH22QJygntv 60TnDkrN5Yh1wlnPkfW72c3!1941873617!-949856145!8091!-1)
Harris, R C.1; Hill, C1; Wise, J A.1

1School of Sports, Exercise & Health Sciences, University College Chichester, Chichester, West Sussex, UK P019 6PE and Natural Alternatives International Inc., 1185 Linda Vista Drive, San Marcos CA 92069, USA

(Sponsor: William J. Kraemer, FACSM)

Carnosine contributes to physico-chemical buffering of H+ in muscle by virtue of its histidine residue which exhibits a pKa of 6.83. Carnosine (β-alanylhistidine) is synthesised in muscle from its 2 constituent amino acids of which β-alanine (β-Ala) appears to be limiting. β-Ala is normally obtained from ex-muscle synthesis or meat ingestion.

PURPOSE
To investigate if β-Ala supplementation increases the capacity to undertake intense exercise normally associated with lactate accumulation.

METHODS
28 healthy male subjects (24-30 years; 65-95kg), active in one or more sport, were supplemented with (a) (4 × 800 mg).d-1 β-Ala for 5w with (4 × 5g).d-1 creatine monohydrate (CrM) during the 5th week (n = 10); (b) (4 × 800mg).d-1 placebo for 5w with (4 × 5g).d-1 CrM over the 5th week (n = 9); (c) (4 × 800mg).d-1 matching placebo for 5w with an additional (4 × 5g).d-1 placebo replacing the CrM during the 5th week (n = 9). Treatments were allocated randomly and the study conducted double blind. Isometric endurance at 50% MVC and power output during a 4 min all-out maximal ergometer exercise (PO4max) were assessed before and at the end of the 5th week.

RESULTS
The mean endurance at 50% MVC and the product of force × time showed a small and equal increase in (a) and (b) relative to (c) though this was not significant. PO4max was increased in (a) >(b) >(c) (38.2, 8.7 and -1.6W, respectively). The contrast of (a) with (b) or (c) was significant (P < 0.05) but not (b) with (c). Most of the gain in PO in (a) occurred during the 1 minute of exercise.

CONCLUSION
Supplementation with β-Ala increases maximal PO during the first minute of maximal dynamic exercise before full cardiovascular adjustment occurs, probably as a result of an increase in H+ buffering by muscle carnosine.



Daily dose of beta-alanine used in study – 3.2 grams
Daily dose of beta-alanine delivered by Body Octane – 3.2 grams



The absorption of orally supplied beta-alanine and its effect on muscle carnosine synthesis in human vastus lateralis – Full Text (http://www.springerlink.com/content/j471337168464032/fulltext.pdf)


The absorption of orally supplied beta-alanine and its effect on muscle carnosine synthesis in human vastus lateralis – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abstract&list_uids=16554972&query_hl=7&itool=pubmed_docsum)

Harris RC, Tallon MJ, Dunnett M, Boobis L, Coakley J, Kim HJ, Fallowfield JL, Hill CA, Sale C, Wise JA.

School of Sports, Exercise and Health Sciences, University College Chichester, Chichester, U.K..

beta-Alanine in blood-plasma when administered as A) histidine dipeptides (equivalent to 40 mg . kg(-1) bwt of beta-alanine) in chicken broth, or B) 10, C) 20 and D) 40 mg . kg(-1) bwt beta-alanine (CarnoSyntrade mark, NAI, USA), peaked at 428 +/- SE 66, 47 +/- 13, 374 +/- 68 and 833 +/- 43 microM. Concentrations regained baseline at 2 h. Carnosine was not detected in plasma with A) although traces of this and anserine were found in urine. Loss of beta-alanine in urine with B) to D) was <5%. Plasma taurine was increased by beta-alanine ingestion but this did not result in any increased loss via urine. Pharmacodynamics were further investigated with 3 x B) per day given for 15 d. Dietary supplementation with I) 3.2 and II) 6.4 g . d(-1) beta-alanine (as multiple doses of 400 or 800 mg) or III) L-carnosine (isomolar to II) for 4 w resulted in significant increases in muscle carnosine estimated at 42.1, 64.2 and 65.8%.





The natural substrate for nitric oxide synthase activity - Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11746209&dopt=Citation)

Alaghband-Zadeh J, Mehdizadeh S, Khan NS, O'Farrell A, Bitensky L, Chayen J.

Department of Clinical Chemistry, Imperial College School of Medicine, Charing Cross Campus, London, UK.

There has been little evidence to indicate that arginine is the natural substrate for generating nitric oxide synthase (NOS) activity. It is now shown that carnosine, which is widely distributed in tissues, is likely to be the true substrate. In tissue sections it gives a stronger NOS reaction than does arginine. Copyright 2001 John Wiley & Sons, Ltd.



High level of skeletal muscle carnosine contributes to the latter half of exercise performance during 30-s maximal cycle ergometer sprinting – Full Text (http://www.jstage.jst.go.jp/article/jjphysiol/52/2/199/_pdf)

High level of skeletal muscle carnosine contributes to the latter half of exercise performance during 30-s maximal cycle ergometer sprinting – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12139778&dopt=Citation)



Buffering capacity of deproteinized human vastus lateralis muscle – Abstract ( http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3968004&dopt=Citation)



Not only does High-Voltage Body Octane use research-backed ingredients, but it also delivers research-backed dosing. Research-backed dosing is one of the major reasons that High-Voltage Body Octane is not a proprietary blend.


I also wanted to include a little bit of info on glucuronolactone. As far as I know, there are no studies done on glucuronolactone as a standalone ergogenic aid in humans. There are a few studies done on energy drinks(i.e. Red Bull) which include glucuronolactone as one of the ingredients. But, those drinks also include other ergogenic aids like caffeine so the research on them is not very conclusive in terms of the role glucuronolactone plays in the results of the studies. I included links on those studies anyway in case anyone wants to check them out. I also included an article written by Androgenic(see last link) that gives more complete info on glucuronolactone’s research, safety, and efficacy. The article is fully referenced.





Glucuronolactone


Efficacy of a 'functional energy drink' in counteracting driver sleepiness – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11897259&dopt=Citation)



The effects of red bull energy drink on human performance and mood – Full Text ( http://www.springerlink.com/content/xxdbgvjy9ke72wbl/fulltext.pdf)

The effects of red bull energy drink on human performance and mood - Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11665810&dopt=Citation)




Beneficial effects of an "energy drink" given to sleepy drivers – Full Text ( http://www.springerlink.com/content/8t8x100htaacpxck/fulltext.pdf)

Beneficial effects of an "energy drink" given to sleepy drivers - Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11310933&dopt=Abstract)




A taurine and caffeine-containing drink stimulates cognitive performance and well-being – Full Text (http://www.springerlink.com/content/6mnudkdx87n9c041/fulltext.pdf)

A taurine and caffeine-containing drink stimulates cognitive performance and well-being – Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11140366&dopt=Abstract)




Glucuronolactone and the Athlete – Article by Shawn Wells(aka Androgenic) (http://www.bodybuilding.com/fun/jrod15.htm)

[NATHAN518]

great info dwm! i have said it a million times...i think beta alanine is THE most underrated supplement...and of course BO has many other excellent synergistic ingredients...

if you need any testers, you know where to find me;)

[UberBerzerker]

Colon Powell, dwm, Colon Powell !!!!!

:p



nice work fo'realz

[IntensityX]

I apologize for being out of the loop but is this a new version of Body Octane?

[dwm230000]

I apologize for being out of the loop but is this a new version of Body Octane?

No, the "High-Voltage" version was released at the end of last year. This is just some of the research behind the product.

[thruxton]

wow. i was just trialing this product and thought i better do some research. a search brought me to your post.

THANKS!

what kind of effect are you getting from this product? i get pretty warm all over, kind of like niacin can do (not flushed though). i think the RDA for niacin is 16mg so i don't think it's this (BO = 25mg).

[warriors]

wow. i was just trialing this product and thought i better do some research. a search brought me to your post.

THANKS!

what kind of effect are you getting from this product? i get pretty warm all over, kind of like niacin can do (not flushed though). i think the RDA for niacin is 16mg so i don't think it's this (BO = 25mg).


That feeling should be from the Beta Alanine in it.

[dwm230000]

wow. i was just trialing this product and thought i better do some research. a search brought me to your post.

THANKS!

what kind of effect are you getting from this product? i get pretty warm all over, kind of like niacin can do (not flushed though). i think the RDA for niacin is 16mg so i don't think it's this (BO = 25mg).


The feeling you are getting is called paresthesia and is from the beta-alanine as warriors mentioned. It is a similar feeling to niacin flush and is not uncommon with beta-alanine use. Most users get it to some degree, especially when first starting Body Octane.

[Coulaid]

Awsome! I love me some BO!

[dwm230000]

great info dwm! i have said it a million times...i think beta alanine is THE most underrated supplement...and of course BO has many other excellent synergistic ingredients...

if you need any testers, you know where to find me;)


We are going to look for some testers for Body Octane and Body Octane/Orotine soon. There will be a new thread for tester applications in a few days.

[deserusan]

i have said it a million times...i think beta alanine is THE most underrated supplement...

Yeah we know :) I will be applying for the BO tester gig. Good post DWM!!!

[NATHAN518]

Yeah we know :) I will be appying for the BO tester gig. Good post DWM!!!
LOL...you noticed?:D

seriously, des, i would love to see you try this out :cool:

[Elliptical Envy]

I'll apply even though I already have some BO/Clout at home.

[dwm230000]

Here is another abstract link to a recent study involving beta-alanine.


Effect of Creatine and ß-Alanine Supplementation on Performance and Endocrine Responses in Strength/Power Athletes - Abstract (http://www.humankinetics.com/IJSNEM/viewarticle.cfm?jid=2jnz7e6d2mar6fzx2uba2qc42qcz84 qs2gaf22RB2vwk&aid=6028&site=2jnz7e6d2mar6fzx2uba2qc42qcz84qs2gaf22RB2vwk)

Jay Hoffman; Nicholas A. Ratamess; Jie Kang; Gerald Mangine; Avery Faigenbaum; Jeffrey Stout
Table of Contents for Vol. 16, Iss. 4

Abstract
The effects of creatine and creatine plus ß-alanine on strength, power, body composition, and endocrine changes were examined during a 10-wk resistance training program in collegiate football players. Thirty-three male subjects were randomly assigned to either a placebo (P), creatine (C), or creatine plus ß-alanine (CA) group. During each testing session subjects were assessed for strength (maximum bench press and squat), power (Wingate anaerobic power test, 20-jump test), and body composition. Resting blood samples were analyzed for total testosterone, cortisol, growth hormone, IGF-1, and sex hormone binding globulin. Changes in lean body mass and percent body fat were greater (P < 0.05) in CA compared to C or P. Significantly greater strength improvements were seen in CA and C compared to P. Resting testosterone concentrations were elevated in C, however, no other significant endocrine changes were noted. Results of this study demonstrate the efficacy of creatine and creatine plus ß-alanine on strength performance. Creatine plus ß-alanine supplementation appeared to have the greatest effect on lean tissue accruement and body fat composition.

[dwm230000]

Another recent study :)


Influence of beta-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity - Abstract (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16868650&dopt=Citation)

Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH, Kim CK, Wise JA.

School of Sports, Exercise & Health Sciences, University of Chichester, Chichester, U.K..

Muscle carnosine synthesis is limited by the availability of beta-alanine. Thirteen male subjects were supplemented with beta-alanine (CarnoSyntrade mark) for 4 wks, 8 of these for 10 wks. A biopsy of the vastus lateralis was obtained from 6 of the 8 at 0, 4 and 10 wks. Subjects undertook a cycle capacity test to determine total work done (TWD) at 110% (CCT(110%)) of their maximum power (W(max)). Twelve matched subjects received a placebo. Eleven of these completed the CCT(110%) at 0 and 4 wks, and 8, 10 wks. Muscle biopsies were obtained from 5 of the 8 and one additional subject. Muscle carnosine was significantly increased by +58.8% and +80.1% after 4 and 10 wks beta-alanine supplementation. Carnosine, initially 1.71 times higher in type IIa fibres, increased equally in both type I and IIa fibres. No increase was seen in control subjects. Taurine was unchanged by 10 wks of supplementation. 4 wks beta-alanine supplementation resulted in a significant increase in TWD (+13.0%); with a further +3.2% increase at 10 wks. TWD was unchanged at 4 and 10 wks in the control subjects. The increase in TWD with supplementation followed the increase in muscle carnosine.

[Carb]

I've noticed a significant positive difference in sleeping patterns with the addition of methylcobalamin vis-a-vis the original BodyOctane. Although this study gave subjects a lot more b-12.


Effects of vitamin B12 on performance and circadian rhythm in normal subjects.
Mayer G, Kroger M, Meier-Ewert K.
Sleep Disorder Unit, Hephata Klinik, Schwatmstadt-Treysa, Germany.

This preliminary study investigates effects of methyl- and cyanocobalamin on circadian rhythms, well-being, alertness, and concentration in healthy subjects. Six women (mean age 35 years) and 14 men (mean age 37 years) were randomly assigned to treatment for 14 days with 3 mg cyano-(CB12) or methylcobalamin (MB12) after 9 days of pre-treatment observation. Levels in the CB12 group increased rapidly in the first, then slowly in the second treatment week, whereas increase in the MB12 group was linear. Urinary aMT6s excretion was reduced by both forms of vitamin B12 over 24 hours with a significant decrease between 0700-1100 hours, whereas urinary excretion of potassium was significantly increased between 0700-1100 hours. Activity from 2300-0700 hours increased significantly under both forms of vitamin B12. Sleep time was significantly reduced under MB12 intake. In this group the change in the visual analogue scales items "sleep quality," "concentration," and "feeling refreshed" between pretreatment and the first week of treatment showed significant correlations with vitamin B12 plasma levels. Cortisol excretion and temperature were not affected by either medication. We conclude that vitamin B12 exerts a direct influence on melatonin. Only MB12 has a positive psychotropic alerting effect with a distribution of the sleep-wake cycle toward sleep reduction.

[RB12]

great info dwm, throughout my logged results I cannot say enough about the quality of results BO produces

[dwm230000]

I've noticed a significant positive difference in sleeping patterns with the addition of methylcobalamin vis-a-vis the original BodyOctane. Although this study gave subjects a lot more b-12.

Good stuff, Carb. Thanks for posting the study.

[Zachattack43]

just a friendly bump of superior supplement people seem to have forgotten about

[riseboi]

what a coinky dink? I justed started the EPM stack a few days ago. :)